DOI: 10.31038/EDMJ.2018241

Abstract

Introduction

Vitamin D and osteocalcin have been reported to affect insulin secretion and sensitivity in the elderly. This study aimed to investigate whether the levels of 25-hydroxyvitamin D [25(OH)D] and osteocalcin were associated with insulin sensitivity in young Korean women.

Methods

One hundred and twenty-eight healthy women (16–38 yrs) were recruited and divided into obese (BMI ≥ 25 kg/m²) and non-obese (BMI < 25 kg/m²) groups according to their body mass index (BMI). The serum 25(OH)D level was measured by radioimmunoassay, total osteocalcin was measured by electrochemiluminescence immunoassay, and carboxylated osteocalcin was measured by enzyme immunoassay. The standard 75-g oral glucose tolerance test was performed, and the metabolic clearance rate was calculated as an index of insulin sensitivity: [MCR = 18.8 – (0.271 × BMI) – (0.0052 × Insulin₁₂₀) – (0.27 × Glucose₉₀)].

Results

The median levels of 25(OH)D were below 20 ng/ml in both obese and non-obese subjects. Obese women had higher serum 25(OH)D (P < 0.01) levels, but the total and carboxylated osteocalcin levels did not differ between the two groups (P > 0.05). Multiple linear regression analysis showed that 25(OH)D was significantly negatively associated with the MCR after adjustment for age, total osteocalcin, and carboxylated osteocalcin (β = -0.333, P < 0.01).

Conclusion

Most of the participants had vitamin D deficiency. A low vitamin D level interferes with conclusions regarding the relationship between osteocalcin and metabolic parameters. Further prospective studies will be needed to confirm the different effects of vitamin D/osteocalcin on insulin sensitivity according to age.

Keywords

25-hydroxyvitamin D; Osteocalcin; Insulin sensitivity

Introduction

Most Koreans have low levels of 25-hydroxyvitamin D [25(OH)D] and a high prevalence of vitamin D insufficiency. The prevalence of vitamin D insufficiency is higher in young adults than in elderly people [1]. Additionally, the prevalence of type 2 diabetes mellitus is rapidly rising. Low insulin sensitivity is associated with type 2 diabetes mellitus [2]. Vitamin D and osteocalcin have been reported to affect insulin secretion and insulin sensitivity [3,4]. Vitamin D deficiency has been associated with impaired insulin secretion in humans and in animal models [5]. The concentration of 25(OH)D was lower in patients with type 2 diabetes mellitus than in nondiabetic controls [6].

Total osteocalcin is synthesized by osteoblasts and undergoes a posttranslational vitamin K-dependent modification in which 3 glutamic acid residues are carboxylated. Total osteocalcin and carboxylated osteocalcin synthesis are directly regulated by 1, 25-dihydroxyvitamin D and parathyroid hormone [7]. In animal models, osteocalcin-deficient mice have obesity, hyperglycemia, glucose intolerance, and insulin sensitivity [4,8]. In humans, serum total osteocalcin is inversely associated with measures of insulin sensitivity and fat mass [3]. In a cross-sectional study of nondiabetic older adults, elevated carboxylated osteocalcin is associated with lower insulin sensitivity [7].

However, increasing numbers of studies have suggested that there is ambiguity and inconsistency between the rodent studies and results in humans. Few studies have confirmed a link between energy metabolism of the adipose tissue and skeleton. There have been multiple studies in young and normoglycemic populations, even in Asians reporting the lack of a relationship or ambiguity between vitamin D/osteocalcin and insulin sensitivity [9–13]. Furthermore, the interaction between vitamin D and insulin sensitivity in the general population has not been adequately determined. Many previous studies on the association of vitamin D/osteocalcin and insulin sensitivity were performed in older adults [6,7]. Therefore, we aimed to evaluate whether a low vitamin D status was associated with insulin sensitivity in young Korean women.

Methods

We performed a survey to identify genetic markers for polycystic ovary syndrome in the Korean population [14]. Subjects were excluded if they had been on medication which had effect on calcium metabolism and vitamin D levels, such as calcium, vitamin D, and anticonvulsant drugs or if they had a condition causing malabsorption of nutrients such as inflammatory bowel disease. Subjects who had been diagnosed with type 2 diabetes mellitus or who had any missing data due to the inadequate sample volume were excluded. Finally, of 2000 women under 40 years of age who voluntarily participated in the survey between 2008 and 2010, we enrolled 128 healthy young women. Throughout the year, we invited volunteers to visit our hospital on the morning after an overnight fast of at least 8 h. Based on their Body Mass Index (BMI), subjects were divided into two groups. The BMI was categorized as < 25 kg/m² or ≥ 25 kg/m², which was suggested as the cut-off point for obesity in Asian populations [15]. Written informed consent was obtained from all participants or their parents if the participants were younger than 18 years of age. The institutional review board of Ewha Womans University Mokdong Hospital approved this study.

Height, weight, and waist circumference were measured. The waist circumference was measured on bare skin at the narrowest indentation between the 10^th rib and iliac crest at midrespiration. BMI, defined as body weight in kilograms divided by the square of height in meters (kg/m²), was used as an index of obesity. The blood pressure was calculated as the mean of two manual sphygmomanometer readings with the patient in the seated position.

We stored the frozen serum samples, which were drawn after overnight fasting, at −80°C until analysis and instantly kept the frozen serum sample at -20°C for measurement. The serum 25(OH)D was measured by radioimmunoassay (Parkard, CA, USA), and the mean inter- and intraassay coefficients of variation (CV) were 7.3% and 8.1%, respectively. Total osteocalcin was measured using an electrochemiluminescence immunoassay (Roche, MA, USA), and the mean inter- and intraassay CVs were 1.5% and 0.7%, respectively. Carboxylated osteocalcin was measured by enzyme immunoassay (Takara Bio, Shiga, Japan), and the mean inter- and intraassay CVs were 1.4% and 3.7%, respectively. The 75-g oral glucose tolerance test (OGTT) was performed in the morning after an 8-hr overnight fast. Blood for measuring the insulin and glucose levels was collected at 0, 30, 60, 90, 120 minutes. Glucose levels were measured by the glucose oxidase method (Beckman Model Glucose Analyzer 2, CA, USA), and insulin levels were measured by radioimmunoassay (BioSource, Nivelles, Belgium). The homeostasis model assessment-estimated insulin resistance (HOMA-IR) index was calculated as the product of the fasting insulin level (mIU/L) and fasting glucose level (mg/dL) divided by 405. The metabolic clearance rate (MCR) was calculated as an index of insulin sensitivity by the Stumvoll Method: [MCR (mL/kg·min) = 18.8 – (0.271 × BMI) – (0.0052 × Insulin₁₂₀) – (0.27 × Glucose₉₀)] [16].

The statistical analyses were performed using SPSS 18.0 software package for Windows (IBM Corporation, Chicago, IL, USA). Because 25(OH)D, total osteocalcin, carboxylated osteocalcin, and MCR had a markedly skewed distribution, logarithmic transformation of these values was performed before regression analysis. Quantitative variables were reported as the means ± standard deviations. Variables with a skewed deviation were reported with medians and interquartile ranges. Two groups with different parameters were compared using Student’s t test or Mann-Whitney U test, depending on the data distribution. To investigate the association of the serum 25(OH)D, total osteocalcin, and carboxylated osteocalcin with insulin sensitivity, multiple linear regression analyses were performed. The considered covariates were age, total osteocalcin, and carboxylated osteocalcin. Statistical significance was defined as P < 0.05.

Results

Table 1 shows the clinical characteristics of the subjects. Age was comparable between obese and non-obese women. The mean BMI (P < 0.01), waist circumference (P < 0.01), systolic blood pressure (P < 0.01), diastolic blood pressure (P < 0.01), and 2-hour post-load glucose (P < 0.01) were higher in obese women than in non-obese women. The MCR was significantly lower, and fasting plasma insulin and HOMA-IR were higher in obese women than in non-obese women (Ps < 0.01, Table 1).

Table 2 shows the bone metabolism parameters of subjects according to BMI. Obese women had higher serum 25(OH)D (P < 0.01) levels; however, the total and carboxylated osteocalcin levels did not differ between the two groups (P > 0.05). The median 25(OH)D levels were below 20 ng/ml in both obese and non-obese subjects (Table 2).

Multiple linear regression analysis showed that 25(OH)D was negatively associated with MCR (β = -0.333, P < 0.01) and positively associated with the 2-hour post-load glucose (β = 0.254, P < 0.01) after adjusting for age, total osteocalcin, and carboxylated osteocalcin (Table 3).

Discussion

In this study, the 25(OH)D levels were low in both obese and non-obese subjects. Most of the participants had vitamin D deficiency. Obese women had higher serum 25(OH)D levels. The total and carboxylated osteocalcin levels did not differ between the two groups. Additionally, a higher 25(OH)D level was negatively associated with insulin sensitivity.

Vitamin D is acquired through diet and cutaneous synthesis in sunlight. It is called vitamin D₃ (cholecalciferol) after its formation in the skin and vitamin D₂ or D₃ when obtained from dietary sources that undergo hydroxylation in the liver, resulting in formation of 25(OH)D, the chief circulating form of vitamin D [17].

In contrast to previous reports [6,18], a higher 25(OH)D level was negatively associated with insulin sensitivity in this study. Hypovitaminosis D has long been suspected as a risk factor for glucose intolerance [19]. Vitamin D status has been inversely associated with diabetes mellitus in epidemiological studies [5,20]. Several clinical intervention studies support that vitamin D supplementation may affect glucose homeostasis or insulin sensitivity [21,22]. Significant improvements in the FPG, insulin, and homeostasis model assessment-insulin sensitivity after treatment with vitamin D were reported in a study of 100 patients with type 2 diabetes mellitus who were between 30 to 70 years old [22]. A potential explanation for the association between a low 25(OH)D level and impaired glucose tolerance has focused on the direct effects of vitamin D on pancreatic ß-cell insulin secretion. Vitamin-D receptors and vitamin D-binding proteins are known to exist in pancreatic tissues. In in vitro studies, 1, 25-dihydroxyvitamin D₃, the active form of vitamin D, induced increased transcription and protein expression of insulin receptors [23].

Table 1. Clinical characteristics of the subjects

Plus-minus values indicate the means ± SD.
Values before parentheses are medians, and values in parentheses are interquartile ranges.
P values were calculated with the Mann-Whitney U test.
P values, non-obese subjects vs obese subjects.
Non-obese: BMI < 25 kg/m²
Obese: BMI ≧ 25 kg/m²
BMI: Body Mass Index
BP: Blood Pressure
FPG: Fasting Plasma Glucose;
2-h PPG: 2-hour Post-Load Glucose
FPI: Fasting Plasma Insulin
2-h PPI: 2-hour Post-Load Insulin
MCR: Metabolic Clearance Rate
HOMA-IR: Homeostasis Model Assessment-Estimated Insulin Resistance

Table 2. Bone metabolism parameters of the subjects

Values before parentheses are medians, and values in parentheses are interquartile ranges.
P values were calculated with the Mann-Whitney U test
P values, non-obese subjects vs obese subjects
Non-obese: BMI < 25 kg/m²
Obese: BMI ≧ 25 kg/m²
25(OH)D: 25-hydroxyvitamin D

Table 3. Association of bone metabolism parameters and insulin sensitivity indices

FPG: Fasting Plasma Glucose
2-h PPG: 2-hour post-load glucose
HOMA-IR: The Homeostasis Model Assessment-Estimated Insulin Resistance
MCR: Metabolic Clearance Rate
25(OH)D: 25-hydroxyvitamin D

The MCR, 25(OH)D, total osteocalcin, and carboxylated osteocalcin were analyzed after log transformation.

In this study, obese women had higher levels of serum 25(OH)D. This is not consistent with the associations reported in previous studies. In the Korea Third National Health and Nutrition Examination Survey of 8,421 men and women, an inverse association between the 25(OH)D level and metabolic syndrome risk, particularly for abdominal obesity, was reported [24]. Obese individuals have low concentrations of 25(OH)D [25]. In a large cohort of 302 healthy adults in the USA, the serum 25(OH)D level was negatively correlated with the BMI and body fat mass [26]. Sequestration of vitamin D in body-fat stores and its consequent reduced bioavailability have been suggested as an explanation for this association [27].

Risk factors for hypovitaminosis D include older age, female sex, lower latitude, winter season, and darker skin pigmentation as well as factors that determine sunlight exposure, such as clothing, cultural practices, and dietary habits. In Canada, young women of Asian and African descent had lower levels of vitamin D than white Canadian women. Additionally, in Australia, Middle Eastern and Asian immigrants had lower vitamin D levels than white Australian and European women [28]. These studies indicate that ethnic differences play a role in the circulating level of serum vitamin D. The sunlight exposure time is different according to the time of year. When we adjusted the sampling time of year because the subjects in our study had a low vitamin D status, there was no effect of the difference of the sampling time on the results. Therefore, the difference in the sampling time may not affect the vitamin D levels in our study.

In general, older age has been suggested as a risk factor for vitamin D insufficiency because cutaneous synthesis of vitamin D₃ declines with age [29]. However, in Korea and Canada, vitamin D insufficiency is more prevalent among young adults than in the older population [1]. In the Korea National Health and Nutrition Examination Survey from 2008, the serum 25(OH)D level increased with age in those between 20–29 and 60–69 years of age in both sexes, and vitamin D insufficiency was most prevalent in the 20–29 age group. These observations may be due to behavioral factors, including an indoor lifestyle, sunscreen use, outdoor activity, and dietary habits [29]. It is unclear why the associations between 25(OH)D and insulin sensitivity and between 25(OH)D and obesity in this study were different from the results of previous reports. Ethnic differences and low vitamin D status in our subjects may provide an explanation for the different relationship of 25(OH)D.

Contrary to the associations seen in previous studies, the total osteocalcin levels did not differ between the obese and non-obese women in this study. As previously described in detail [30], total osteocalcin is the most abundant noncollagenous protein of the bone matrix, and it has been used as a marker of bone formation. Carboxylated Gla residues are related to calcium and hydroxyapatite binding, allowing for deposition of total osteocalcin in mineralized bone matrix. Uncarboxylated osteocalcin is more easily released into the circulation; however, both the carboxylated and uncarboxylated forms can be detected in blood.

A study in elderly Swedish men showed that the plasma total osteocalcin was a strong negative predictor of fat mass and plasma glucose [31]. Additionally, in the investigation of postmenopausal women, the serum total osteocalcin levels were shown to have a statistically significant negative- correlation with BMI [32]. Total osteocalcin was particularly inversely related to visceral obesity in Korean obese and overweight men [33]. Additionally, the relationship between vitamin D/osteocalcin and insulin sensitivity was weak in young adults [9–12].

The serum total osteocalcin level was significantly negatively correlated with FPG and Hemoglobin A1c in both men and postmenopausal women with type 2 diabetes mellitus [34,35]. Additionally, hyperglycemia influences the response of osteoblasts to parathyroid hormone and 1, 25-dihydroxyvitamin D [36]. In animal models, chronic hyperglycemia decreased total osteocalcin [37]. Circulating levels of total osteocalcin were lower in animals on a high-fat diet, with a larger reduction in those that were under-expressing the insulin receptor [38]. In some studies, the serum total osteocalcin level had no correlation with insulin sensitivity [39,40]. Of note, the total or carboxylated osteocalcin levels were not associated with insulin sensitivity indices in our study.

In nondiabetic older adults, an elevated carboxylated osteocalcin level was associated with lower insulin sensitivity [7]. However, in the present study, carboxylated osteocalcin did not differ between the obese and non-obese groups. Levels of carboxylated and uncarboxylated osteocalcin are influenced by the vitamin K status, while the total circulating concentration of osteocalcin is independent of vitamin K [30]. Factors influencing the levels of carboxylated osteocalcin, such as the vitamin K status, should be considered in explaining the relationship of carboxylated osteocalcin with obesity and insulin sensitivity. In this study, the vitamin K status was not measured. The proportion of carboxylated osteocalcin was lower than in previous studies. The difference of the standardization of methods for measuring osteocalcin, sample stability or handling, and the biological variability may affect the levels of carboxylated osteocalcin. We used an enzyme immunoassay to measure carboxylated osteocalcin; however, a two-sited immunofluorometric assay was used in a previous study [12].

With this study population being limited to young Korean women, confounding factors affecting bone metabolism according to ethnicity and age could be controlled. However, there are some limitations in this study. First, due to the small sample size, generalization of the results is difficult. Therefore, additional research is necessary to determine the patterns in other age and ethnic groups. Second, measurements of physical activity were not made in this study. Comparison of this information between the obese and non-obese groups would be helpful to further understand the mechanism behind the differences in 25(OH)D status between the two young age groups. Finally, most subjects in this study had vitamin D deficiency [defined as 25(OH)D of less than 20 ng/ml, n = 102 (80%)] or insufficiency [25(OH)D from 20 to less than 30 ng/ml, n = 21 (16%)]. Considering that the obese group had only 9% (n = 5) with a sufficient 25(OH)D level, it was difficult to analyze the relationship between 25(OH)D and obesity. Additionally, other bone turnover markers and vitamin K statuses should be considered to confirm the status of bone turnover in these vitamin D-deficient subjects. The results may reflect overall bone formation because we did not measure other bone formation markers, especially the bone resorption markers.

Conclusion

In conclusion, the levels of 25(OH)D were low in our subjects. Unlike in previous reports, a higher 25(OH)D level was negatively associated with insulin sensitivity in young Korean women, suggesting that the low vitamin D levels interfere with conclusions on the relationship between osteocalcin and metabolic parameters. Further studies on subjects of different ages and a prospective study in young women of the same age will be needed to confirm the different effects of vitamin D/osteocalcin on insulin sensitivity according to age.

Acknowledgement

We appreciate Yoon Jeong Lee R.N. for her help in recruitment and testing of the subjects.

References

1. Choi HS (2013) Vitamin d status in Korea. Endocrinol Metab (Seoul) 28: 12–16. [crossref]
2. Abdul-Ghani MA, Williams K, DeFronzo R, Stern M (2006) Risk of progression to type 2 diabetes based on relationship between postload plasma glucose and fasting plasma glucose. Diabetes Care 29: 1613–1618.
3. Pittas AG, Harris SS, Eliades M, Stark P, Dawson-Hughes B (2009) Association between serum osteocalcin and markers of metabolic phenotype. J Clin Endocrinol Metab 94: 827–832.
4. Lee NK, Sowa H, Hinoi E, Ferron M, Ahn JD, et al. (2007) Endocrine regulation of energy metabolism by the skeleton. Cell 130: 456–469. [crossref]
5. Teegarden D, Donkin SS (2009) Vitamin D: emerging new roles in insulin sensitivity. Nutr Res Rev 22: 82–92. [crossref]
6. Pittas AG, Lau J, Hu FB, Dawson-Hughes B (2007) The role of vitamin D and calcium in type 2 diabetes. A systematic review and meta-analysis. J Clin Endocrinol Metab 92: 2017–2029. [crossref]
7. Shea MK, Gundberg CM, Meigs JB, Dallal GE, Saltzman E, et al. (2009) Gamma-carboxylation of osteocalcin and insulin resistance in older men and women. Am J Clin Nutr 90: 1230–1235. [crossref]
8. Ferron M, Hinoi E, Karsenty G, Ducy P (2008) Osteocalcin differentially regulates ß cell and adipocyte gene expression and affects the development of metabolic diseases in wild-type mice. Proc Natl Acad Sci U S A 105: 5266–5270.
9. Viljakainen H, Ivaska KK, Paldánius P, Lipsanen-Nyman M, Saukkonen T, et al. (2014) Suppressed bone turnover in obesity: a link to energy metabolism? A case-control study. J Clin Endocrinol Metab 99: 2155–2163. [crossref]
10. Paldanius P, Ivaska K, Hovi P, Andersson S, Väänänen H, et al. (2012) The effect of oral glucose tolerance test on serum osteocalcin and bone turnover markers in young adults. Calcif Tissue Int 90: 90–95.
11. Paldánius PM, Ivaska KK, Hovi P, Andersson S, Eriksson JG, et al. (2013) Total and carboxylated osteocalcin associate with insulin levels in young adults born with normal or very low birth weight. PLoS One 8: 63036. [crossref]
12. Knapen MH, Schurgers LJ, Shearer MJ, Newman P, Theuwissen E, et al. (2012) Association of vitamin K status with adiponectin and body composition in healthy subjects: uncarboxylated osteocalcin is not associated with fat mass and body weight. Br J Nutr 108: 1017–1024. [crossref]
13. Zhou M, Ma X, Li H, Pan X, Tang J, et al. (2009) Serum osteocalcin concentrations in relation to glucose and lipid metabolism in Chinese individuals. Eur J Endocrinol 161: 723–729.
14. Lee H, Oh J-Y, Sung Y-A, Chung H, Kim H-L, et al. (2015) Genome-wide association study identified new susceptibility loci for polycystic ovary syndrome. Hum Reprod 30: 723–731.
15. Organization WH (2000) The Asia-Pacific perspective: redefining obesity and its treatment. Sydney: Health Communications Australia.
16. Stumvoll M, Mitrakou A, Pimenta W, Jenssen T, Yki-Järvinen H, et al. (2000) Use of the oral glucose tolerance test to assess insulin release and insulin sensitivity. Diabetes care 23: 295–301.
17. Holick MF (2007) Vitamin D deficiency. N Engl J Med 357: 266–281. [crossref]
18. Mattila C, Knekt P, Männistö S, Rissanen H, Laaksonen MA, et al. (2007) Serum 25-hydroxyvitamin D concentration and subsequent risk of type 2 diabetes. Diabetes Care 30: 2569–2570. [crossref]
19. Chiu KC, Chu A, Go VL, Saad MF (2004) Hypovitaminosis D is associated with insulin resistance and beta cell dysfunction. Am J Clin Nutr 79: 820–825. [crossref]
20. Scragg R, Sowers M, Bell C (2004) Serum 25-hydroxyvitamin D, diabetes, and ethnicity in the Third National Health and Nutrition Examination Survey. Diabetes care 27: 2813–2818.
21. Pittas AG, Harris SS, Stark PC, Dawson-Hughes B (2007) The effects of calcium and vitamin D supplementation on blood glucose and markers of inflammation in nondiabetic adults. Diabetes care 30: 980–986.
22. Talaei A, Mohamadi M, Adgi Z (2013) The effect of vitamin D on insulin resistance in patients with type 2 diabetes. Diabetol Metab Syndr 5: 8. [crossref]
23. Maestro B, Campión J, Dávila N, Calle C (2000) Stimulation by 1,25-dihydroxyvitamin D3 of insulin receptor expression and insulin responsiveness for glucose transport in U-937 human promonocytic cells. Endocr J 47: 383–391.
24. Ford ES, Ajani UA, McGuire LC, Liu S (2005) Concentrations of serum vitamin D and the metabolic syndrome among U.S. adults. Diabetes Care 28: 1228–1230. [crossref]
25. Konradsen S, Ag H, Lindberg F, Hexeberg S, Jorde R (2008) Serum 1,25-dihydroxy vitamin D is inversely associated with body mass index. Eur J Nutr 47: 87–91. [crossref]
26. Parikh SJ, Edelman M, Uwaifo GI, Freedman RJ, Semega-Janneh M, et al. (2004) The relationship between obesity and serum 1, 25-dihydroxy vitamin D concentrations in healthy adults. J Clin Endocrinol Metab 89: 1196–1199.
27. Rajakumar K, de las Heras J, Chen TC, Lee S, Holick MF, et al. (2011) Vitamin D status, adiposity, and lipids in black American and Caucasian children. J Clin Endocrinol Metab 96: 1560–1567. [crossref]
28. Mithal A, Wahl DA, Bonjour JP, Burckhardt P, Dawson-Hughes B, et al. (2009) Global vitamin D status and determinants of hypovitaminosis D. Osteoporos Int 20: 1807–1820. [crossref]
29. Choi HS, Oh HJ, Choi H, Choi WH, Kim JG, et al. (2011) Vitamin D insufficiency in Korea-a greater threat to younger generation: the Korea National Health and Nutrition Examination Survey (KNHANES) 2008. J Clin Endocrinol Metab 96: 643–651.
30. Patti A, Gennari L, Merlotti D, Dotta F, Nuti R (2013) Endocrine actions of osteocalcin. Int J Endocrinol 2013: 846480. [crossref]
31. Kindblom JM1, Ohlsson C, Ljunggren O, Karlsson MK, Tivesten A, et al. (2009) Plasma osteocalcin is inversely related to fat mass and plasma glucose in elderly Swedish men. J Bone Miner Res 24: 785–791. [crossref]
32. Im JA, Yu BP, Jeon JY, Kim SH (2008) Relationship between osteocalcin and glucose metabolism in postmenopausal women. Clin Chim Acta 396: 66–69. [crossref]
33. Kim SH, Lee JW, Im JA, Hwang HJ (2010) Serum osteocalcin is related to abdominal obesity in Korean obese and overweight men. Clin Chim Acta 411: 2054–2057. [crossref]
34. Fernández-Real JM, Izquierdo M, Ortega F, Gorostiaga E, Gomez-Ambrosi J, et al. (2009) The relationship of serum osteocalcin concentration to insulin secretion, sensitivity, and disposal with hypocaloric diet and resistance training. J Clin Endocrinol Metab 94: 237–245.
35. Kanazawa I, Yamaguchi T, Yamamoto M, Yamauchi M, Kurioka S, et al. (2009) Serum osteocalcin level is associated with glucose metabolism and atherosclerosis parameters in type 2 diabetes mellitus. J Clin Endocrinol Metab 94: 45–49.
36. Horiuchi T, Kazama H, Araki A, Inoue J, Hosoi T, et al. (2004) Impaired gamma carboxylation of osteocalcin in elderly women with type II diabetes mellitus: relationship between increase in undercarboxylated osteocalcin levels and low bone mineral density. J Bone Miner Metab 22: 236–240.
37. Botolin S, McCabe LR (2006) Chronic hyperglycemia modulates osteoblast gene expression through osmotic and non-osmotic pathways. J Cell Biochem 99: 411–424.
38. Wei J, Ferron M, Clarke CJ, Hannun YA, Jiang H, et al. (2014) Bone-specific insulin resistance disrupts whole-body glucose homeostasis via decreased osteocalcin activation. J Clin Invest 124: 1–13.
39. Lu C, Ivaska KK, Alen M, Wang Q, Törmäkangas T, et al. (2012) Serum osteocalcin is not associated with glucose but is inversely associated with leptin across generations of nondiabetic women. J Clin Endocrinol Metab 97: 4106–4114.
40. Gower BA, Pollock NK, Casazza K, Clemens TL, Goree LL, et al. (2013) Associations of total and undercarboxylated osteocalcin with peripheral and hepatic insulin sensitivity and ß-cell function in overweight adults. J Clin Endocrinol Metab 98: 1173–1180.