Article Page

DOI: 10.31038/JCRM.2019252

Abstract

Objectives: Focal Segmental Glomerulosclerosis (FSGS) is a progressive glomerular disease. The pathogenesis of this disease, however, remains unclear. Here, we attempted to identify key candidate genes in FSGS through stringent bioinformatic analysis.

Methods: We systematically searched the Gene Expression Omnibus database for gene expression microarrays derived from human glomeruli tissues with FSGS. First, we identified differentially expressed genes (DEGs) by using the Limma package in R. Then, we subjected these DEGs to Gene Ontology (GO) analysis for further analysis. Finally, we constructed Protein–Protein Interaction networks (PPI) through four different methods and performed intersection analysis to further refine our results. 

Results: A total of 627 DEGs were identified between the FSGS and control groups, among which 534 were up-regulated and 93 were down-regulated. GO analysis revealed that the DEGs were enriched in mRNA processing, cell adhesion molecule binding, and cadherin binding. Furthermore, via PPI, 7 DEGs overlapped in the four groups constructed through different analytical approaches. We also validated the overlapped 7 hub genes in in vitro experiments, including RBM5 and HNRNPF, with potentially important roles in the development of FSGS.

Conclusion: Our study provides a valuable resource for novel biomarkers and therapeutic targets for FSGS.

Keywords

Focal segmental glomerulosclerosis; Bioinformatic analysis; RBM5; HNRNPF

Introduction

Focal Segmental Glomerulosclerosis (FSGS) is a primary glomerular disease that manifests with heavy proteinuria [1]. It is the leading cause of the development of end-stage renal disease. Typically, FSGS lesions present a segmental manifestation that includes parietal cell migration, hyaline deposition, capillary collapse, and intracapillary thrombi.  Recent studies suggest that podocyte injury may play a key role in FSGS lesions [2]. Injury and loss of podocytes result in foot process effacement and protein loss [3]. However, the pathogenesis of FSGS remains unclear, and the present diagnostic and therapeutic methods for this disease remain inadequate. Oxidative stress has been implicated in the development and progression of this FSGS [4,5]. Nuclear factor E2-related factor 2 (Nrf2) is a transcription factor that can potently induce the production of numerous antioxidants and prevent the generation of oxidative stress in renal fibrosis and inflammation [6–8]. Furthermore, apoptosis and the renin–angiotensin system are strongly involved in FSGS-related injury
[9–12]. Nevertheless, a considerable amount of important FSGS genes remain unidentified given the lack of global analysis.

With the development of bioinformatic analysis technology, gene expression profiling analysis has been increasingly used to explore molecular mechanisms and identify novel biomarkers in various diseases [13–16]. Bioinformatic analysis is mainly used to predict novel diagnostic biomarkers and therapeutic targets associated with tumors, such as bladder cancer [17], meningioma [18], and hepatocellular carcinoma [19]. The application of bioinformatic analysis in renal diseases, such as renal cell carcinoma [20], lupus nephritis [21], IgA nephropathy [22], and chronic kidney disease [23], has begun to develop gradually. However, up to now, no study has subjected FSGS to bioinformatic analysis. Thus, exploring the underlying crucial genes and effective therapeutic targets for FSGS through bioinformatic analysis is necessary.

In this study, we downloaded the gene expression profile datasets of FSGS from the Gene Expression Omnibus (GEO) database and investigated Differentially Expressed Genes (DEGs) between FSGS and control samples by using the Limma package in R. We performed Gene Ontology (GO) enrichment analysis for all DEGs. In addition, we constructed protein–protein interaction (PPI) networks, identified novel hub genes, and validated them in in vitro experiments. Our study aimed to predict novel diagnostic biomarkers and potential therapeutic targets for FSGS.

Materials and Methods

Data collection

Gene expression profiles were retrieved from NCBI’s GEO database (http://www.ncbi.nlm.nih.gov/geo/) by using the key words “focal segmental glomerulosclerosis” with the following criteria: 1) the study type is expression profiling by array, 2) the attribute name is tissue, 3) the organism of interest is Homo sapiens, and 4) the platform used is the Affymetrix Human Genome U133A Array. Ultimately, on the basis of the above criteria, we selected dataset GSE47185 of FSGS. Original CEL files were used for further bioinformatic analysis.

Data preprocessing

CEL files were normalized and converted to expression profiles by using the Affy package of R [24]. In brief, the original data were read using the Affy Bioconductor package and preprocessed for normalization through the robust multiarray analysis method, which includes background correction, normalization, expression calculation and batch effects removal. After obtaining the gene expression value, genes were annotated with the hgu133A.db and annotate software packages.

DEG analysis

The Limma package of R [25] was used to analyze DEGs after preprocessing. The linear fit method, Bayesian analysis, and t-test algorithm were used to calculate the P and FC values. DEGs were screened by setting a cut-off value of |log 2 Fold Change (FC)| > 1 and P < 0.05. The ggplot2 software package was used to visualize results. Moreover, to identify and visualize the DEGs between FSGS and normal samples, we generated a heat map of the top 10% DEGs by using the heatmap package (Version 1.0.8).

GO enrichment analysis for DEGs

The GO consortium includes three independent branches: Biological Process (BP), Cellular Component (CC), and Molecular Function (MF). In this study, we subjected the identified DEGs to GO enrichment analysis by using R and the clusterProfiler package [26]. P < 0.05 was used as the threshold for the identification of significant GO terms.

PPI Network Construction

To explore the relationships among the top 30% DEGs, we used the online tool STRING (Search Tool for the Retrieval of Interacting Genes/Proteins; http://string.embl.de/) database for the construction of PPI networks. The minimum required interaction score of 0.4 was used as the significant cut-off threshold. Then, the obtained PPI interaction networks were visualized by using Cytoscape software (version 6.3).

Hub-gene screening

On the basis of the STRING results, we introduced the four methods Degree, EPC, Maximal Clique Centrality (MCC), and DMNC [27] to rank the importance of nodes in the PPI networks and to further identify hub genes from the top 30% DEGs. Nodes with high centrality scores were considered hub genes. We applied the R package Venn diagram (version1.6.17, https://cran.r-project.org/web/packages/VennDiagram/) [28] to identify overlapping DEGs among these hub genes.

Degree (Deg(v) = |N(v)|) is a computing tool in Cytoscape software [29]. The default filter “in and out” was between 7 and 42 in the present study.

MCC is a topological analysis method in CytoHubba [27]. Given a node v, the MCC of v is defined as MCC(v) = ∑CÎS(v)(|C|−1)!.

Maximum Neighborhood Component (MNC) is another computing tool in cytoHubba. MNC(v) = |V(MC(v))|, where MC(v) is a maximum connected component of G[N(v)], and G[N(v)] is the induced subgraph of G by N(v). On the basis of MNC, Lin et al. proposed that DMNC(v) = |E(MC(v))|/ |V(MC(v))| ε , where ε = 1.7.

Cell culture

Conditionally immortalized human podocytes (LY893) were kindly provided by Dr. Lan Ni and Moin Saleem(Bristol, U.K.). Podocytes were cultured in RPMI 1640 medium(Gibco)supplemented with 10% Fetal Bovine Serum (Gibco) and 1% Insulin-Transferrin-Selenium(Invitrogen) at 33°C under 5% CO2 for propagation, then were thermo switched to 37°C under 5% CO2 when at 60% confluency for differentiation. The differentiated podocytes were incubated with adriamycin to construct an in vitro model for FSGS [30].

Quantitative Real-Time PCR

Total RNA was extracted using the RNeasy Plus Mini Kit (BioTeke RP1202) in accordance with the manufacturer’s instructions. The cDNA was obtained by reverse transcription, amplified and detected using a SYBR Green Supermix kit (Takara). Then, a BIO-RAD CFX-96 Real-Time PCR system (Bio-Rad) was used for PCR analysis under the following conditions: 95 °C for 3 min, followed by 40 cycles of 95 °C for 10 s and 51 °C for 30 s. The primer sequences used for PCR are listed in Table 1. Statistical differences were determined by Student’s t-test using R “ggpubr” package(version 0.1.8, https://CRAN.R-project.org/package=ggpubr).

Table 1. Primer sequences for RT-PCR.

Gene

Forward primer

Reverse primer

FUS

5’ GCAGGAGTTTGTGGAGTG 3’

5’ TGAGTACAGGCAGGATGTG 3’

DHX15

5’ CTTTACAAGCAGGGACTA 3’

5’ TCAAGAACAGTAGAGGGAT 3’

PRPF31

5’ TGTCGGGCTTCTCGTCTA 3’

5’ CACCTTCCCTTCTGTGCTCT 3’

PQBP1

5’ CAAGAAGGCAGTAAGCCGAAAG 3’

5’ TGTGGTGTCAGCGCCAGTC 3’

RBM5

5’ GGTGCGAAATGGAGATGA 3’

5’ AGAGTTGCTGGTGCCTGA 3’

HNRNPR

5’ AAGTCCCACAGAACCGAGAT 3’

5’ AACCCTGAGAAGAACTGAACAA 3’

TRA2B

5’ CACATACGCCAACACCAG 3’

5’ TCCTCCACCTCCTCCTCT 3’

GAPDH

5’ CTTTGGTATCGTGGAAGGACTC 3’

5’ GTAGAGGCAGGGATGATGTTCT 3’

Results

DEGs identification

This dataset GSE47185 contains the mRNA expression profiles of 13 FSGS samples and 14 control samples (normal tissue of renal tumor excision). Under the threshold of |log 2 fold change (FC)| > 1 and adj.P value < 0.05, 627 DEGs were identified between the FSGS and control groups. These DEGs included 534 up-regulated and 93 down-regulated DEGs. The results of expression-level analysis are presented as a volcano plot in Fig. 1A. As shown in Table 2, RPS4Y1, PLPP3, DDX3Y, SART3, TCF4, TROVE2, IQGAP1, MBP, CALD1, and RBFOX2 are the 10 most significantly up-regulated genes, whereas CYP4A11, FOSB, EGR1, G6PC, ALB, CTSZ, PPP3R1, XIST, PCK1, and HPGD are the 10 most significantly down-regulated genes. The more information of all DEGs is listed in supplementary Table 1.

Fig1

Figure 1.
Visualization of DEGs
A, The volcano plot of differentially expressed genes between FSGS and healthy tissues. The red plots represent up-regulated genes, green plots represent down-regulated genes, while grey plots represent non-significant genes. The volcano plot was constructed using the ggplot2 package of the R language; B, A heatmap of the top 10% DEGs. The horizontal axis denotes the different samples, and the vertical axis denotes different DEGs.blue, normal samples; red, FSGS samples; purple clusters represent up-regulated DEGs and green clusters represent downregulated DEGs. Color key represents the Z-score based on the Gene expression value.

Table 2. The Most Significant 10 Up-Regulated Genes And Down-Regulated Genes.

Gene Symbols

Log FC

Average Expression level

Adj.P. Value

RPS4Y1

2.569009

9.570289

1.11E-02

PLPP3

2.485089

9.251834

7.13E-07

DDX3Y

2.478844

7.003028

1.62E-02

SART3

2.470577

7.660357

5.82E-10

TCF4

2.41899

7.335133

1.27E-09

TROVE2

2.355365

9.457713

7.39E-07

IQGAP1

2.290646

8.145384

2.19E-08

MBP

2.255968

7.587945

3.91E-10

CALD1

2.192563

8.074506

2.21E-09

RBFOX2

2.164463

8.698168

8.22E-09

CYP4A11

-2.41227

9.128665

1.42E-05

FOSB

-2.35626

8.074382

4.53E-07

EGR1

-2.18278

10.28014

1.01E-07

G6PC

-2.07454

6.566343

5.57E-04

ALB

-2.0735

9.571928

1.21E-03

CTSZ

-1.91281

8.256097

3.49E-08

PPP3R1

-1.88153

7.627366

1.74E-06

XIST

-1.86386

7.615666

1.50E-02

PCK1

-1.7723

10.99439

2.73E-04

HPGD

-1.7174

10.07267

3.87E-06

The heatmaps of the top 10% DEGs are shown in Fig. 1B. The data are presented in a matrix format, in which rows represent individual genes, and columns represent individual samples. The purple and green colors indicate up-regulated and down-regulated genes, respectively. The hierarchy cluster analysis indicated that FSGS and control groups could be distinguished from each other on the basis of their different expression patterns.

Functional enrichment analysis

To reveal the biological functions of DEGs, we used the clusterProfiler package for GO analysis. We set adj.P value < 0.01 to identify significantly enriched GO terms. The top eight GO terms for the DEGs enriched in the BP, CC, and MF are shown in Figure 2. The DEGs were mainly involved in GO terms that included mRNA processing, regulation of mRNA metabolic process, antigen processing and presentation of exogenous antigen, focal adhesion, cell adhesion molecule binding, cadherin binding, and actin binding. Among these terms, the MFs related to focal adhesion (GO:0005925) [31,32], cell adhesion molecule (GO:0050839) [33,34], cadherin (GO:0045296) [35], and actin binding (GO:0003779) [36] were all confirmed to be involved in glomerulosclerosis. Detailed information on the top eight GO terms is shown in Table 3.

JCRM_Zhongying Huang_F2

Figure 2. The 8 most significant enriched GO terms of DEGs
The adj.P value< 0.01 was used as the threshold for the identification of significant GO terms. The Gene ontology covers the biological process, cellular component, and molecular function.The horizontal axis represents the gene counts, the vertical axis represents GO terms.Green column graphs represent biological process(BP) GO term; orange column graphs represent cellular component(CC) GO term; and blue column graphs represent molecular function (MF) GO term.

Table 3. The Most Significantly Enriched GO Terms In BP ,CC and MF.

JCRM_Zhongying-Huang_F5

PPI Network Construction and Hub-Gene Screening

The major part of the constructed PPI network is presented in Figure 3A. To further reduce the scope for analysis, we analyzed the PPI network by using the four analysis methods in CytoHubba based on the R package Venn diagram. We used the top ranked 20 DEGs to identify seven overlapping hub genes screened through the four CytoHubba methods (Degree, EPC, MCC, and DMNC) in cytoscape software (Figure 3B–F). The seven overlapping hub genes included FUS, DHX15, PRPF31, PQBP1, RBM5, HNRNPR, and TRA2B. Strikingly, the identified hub DEGs in our study have never been reported in literature related to FSGS. In addition, these genes simultaneously ranked to the high position by the four different CytoHubba methods suggests they may play important roles in the development of FSGS.

Fig3

Figure 3: Protein-protein interaction (PPI) networks of DEGs and screening of hub genes
A: The major part of PPI network; B: Venn diagram of differentially expressed genes based on four screening methods including “Degree”, “EPC”, “MCC”, and “DMNC”; C: PPI of DEGs screened by the method “Degree” in Cytohubba; D: PPI of DEGs screened by the method “EPC” in Cytohubba; E: PPI of DEGs screened by the method “MCC” in Cytohubba; F: PPI of DEGs screened by the method “DMNC” in Cytohubba. The depth of red represents the rank of the hub genes.

Gene Expression Validation in In -Vitro Experiments

We validated the 7 top ranked hub genes expression in the FSGS model in vitro (Figure 4). Quantitative real-time PCR indicated that the mRNA levels of FUS, DHX15, PQBP1, RBM5, and HNRNPR were up-regulated after they were stimulated by adriamycin (ADR). The changes in PRPF31 and TRA2B mRNA were statistically insignificant. Except for PRPF31 and TRA2B, the changes in all of the other gene expression levels were consistent with the bioinformatic analysis results (Supplementary Table 1), with the accordance rate reaching 70% approximately.

R Graphics Output

Figure 4. In vitro validation for the novel hub genes
Adriamycin (ADR) (0.125 ug/ml) was used to stimulate confluent conditionally immortalized human podocytes (LY893) for 0 (control) and 48 h. The mRNA expression levels of 7 novel top hub genes were measured by quantitative real-time PCR. The mRNA expression levels of the target genes were normalized to that of GAPDH. The data in three separate experiments were presented as mean ± SD (n=3). *Significantly changed expression levels in ADR-stimulated cells compared with the controls (P<0.05). N.S., no significant difference (P>0.05)

Extended information on Potential Hub Genes

On the basis of the above results, we used the abbreviations of the seven hub genes and FSGS as keywords to search the NCBI database for identifying the potential relationship between these hub genes and FSGS. Search results revealed that the seven hub genes have never been reported in literature related to FSGS. Then, we carefully collated information relevant to the biological functions and signaling pathways that involve these hub genes on Gene Cards website (https://www.genecards.org). The results indicate that heterogeneous nuclear ribonucleoprotein F (HNRNPF) is closely related to Nrf2 gene expression, renal angiotensinogen gene expression, the TGF-β1 signaling pathway, and oxidative stress. Moreover, RNA-binding motif protein 5 (RBM5) is involved in apoptosis induction in many tumors. Ultimately, in accordance with the accepted pathogenesis of FSGS, we selected HNRNPF and RBM5 as representative targets for further discussion. Extended information on HNRNPF and RBM5 are shown in Table 4.

Table 4. Extended Information of The Potential MN-Related Hub Genes.

Gene

Function

Disease/Cells

DOI

Authors

HNRNPF

Stimulates renal Ace-2 gene expression and prevents TGF-β1-induced kidney injury

Diabetes

10.1007/s00125-015-3700-y

Lo CS, Shi Y, Chang SY

 

Mediate renal angiotensinogen gene expression and prevention of hypertension and kidney injury

Diabetes

10.1007/s00125-013-2910-4

 

Abdo S, Lo CS, Chenier I

 

Inhibits Nrf2 Gene Expression

Diabetic mice

10.1210/en.2016-1576

Ghosh A, Abdo S, Zhao S

 

Against oxidative stress

Diabetic mice

10.2337/db16-1588

Lo CS, Shi Y, Chenier

 

Suppresses angiotensinogen gene expression

Diabetic mice

10.2337/db11-1349

Lo CS, Chang SY, Chenier I

 

Modulate the alternative splicing of the apoptotic mediator Bcl-x

Human HeLa cells

10.1074/jbc.M501070200

GarneauD,Revil T, Fisette JF

 

Modulates angiotensinogen gene expression

Diabetes

10.1681/ASN.2004080715

Wei CC, Guo DF, Zhang SL

RBM5

Inhibition of Wnt/β-catenin signaling and induction of apoptosis

Gliomas

10.1186/s12957-016-1084-1

Jiang Y, Sheng H, Meng L

 

Impacts cell proliferation and apoptosis

Lung cancer

10.1615/JEnvironPatholToxicolOncol.2017019366

Prabhu VV, Devaraj N

 

Regulates the activity of Wnt/β-catenin signaling

Alveolar epithelial injury

10.3892/or.2015.3828

Hao YQ, Su ZZ, Lv XJ

 

Promotes caspase activation

Human neuronal cells

10.1038/jcbfm.2014.242

Jackson TC, Du L,Janesko-Feldman K

 

Promotes neuronal apoptosis

Spinal cord injury

10.1016/j.biocel.2014.12.020

Zhang J, Cui Z, Feng G

 

Inhibits cell growth and induces apoptosis

Lung adenocarcinoma

10.1186/1477-7819-10-160

Shao C, Zhao L, Wang K

Discussion

Bioinformatics is a newly developed interdisciplinary subject that combines biological science and computer science. Over the past few years, a growing body of research has used gene expression profiles to explore key genes in the pathogenesis of numerous diseases [15,16,40,41]. To our knowledge, our study is the first work that subjected FSGS to bioinformatic analysis. We identified 627 DEGs between the FSGS and control groups. These DEGs included numerous DEGs that have not been previously reported to be involved in FSGS. Then, we predicted DEG functions on the basis of GO annotations. The GO terms we identified included focal adhesion [31,32], cell adhesion molecule [33,34], cadherin [35], and actin binding [36]. These processes are associated with glomerulosclerosis. For example, the genetic deletion of Epb41l5, a podocyte-specific focal adhesome component, results in podocyte detachment, severe proteinuria, and focal segmental glomerulosclerosis development [31]. In immortalized human podocytes, the overexpression of R431C mutant ANLN, an F-actin binding cell cycle gene, enhances podocyte motility [36]. Next, we identified seven overlapping hub genes by constructing PINs through four different analytical methods. Through an accurate search of the NCBI database, we identified HNRNPF and RBM5 as the representative targets for further elaboration.

HNRNPF is a protein-coding gene associated with gene expression. However, no research has been conducted on the role and mechanisms of HNRNPF in FSGS. In this work, we found that HNRNPF is an important DEG among the seven overlapping hub genes in the PPI networks. In addition, HNRNPF is deeply involved with Nrf2 [42], a renal angiotensinogen gene that is expressed in the kidney [43]. Furthermore, in diabetic mice, HNRNPF participates in the TGF-β1 signaling pathway [44] and oxidative stress [45]. These genes and pathways have been confirmed to play vital roles in the pathogenesis of FSGS [4,11,46]. One research suggested that osthole could improve FSGS by activating the Nrf2 antioxidant pathway [47]. TGF-β1 reduces WT1 expression in mouse podocytes and cultured human podocytes before overt glomerulosclerosis begins [46]. In addition, damage to podocytes stimulates TGF-β1 and TGF-βIIR expression in glomerular epithelial cells; this effect eventually leads to extracellular matrix overproduction [48]. On the basis of our analytical results, we conclude that HNRNPF likely participates in FSGS through oxidative stress-associated genes and pathways and is a potential biomarker for this disease.

RBM5 is a nuclear RNA-binding protein that is often genetically deleted in renal cancer49. Unfortunately, the role of RBM5 in FSGS remains unreported. In our study, we identified RBM5 as an up-regulated hub gene in FSGS. Moreover, RBM5 actively participates in apoptosis induction in tumors [50,51]. Apoptosis-induced podocyte damage is a key factor in the pathogenesis of FSGS [12,52]. On the basis of previous findings combined with our present bioinformatic analysis results, we speculate that RBM5 may participate in apoptosis promotion during FSGS progression.

Conclusion

Our study provides a fast, powerful, and effective strategy for the discovery of novel diagnostic biomarkers and therapeutic targets for FSGS. Our results suggest that HNRNPF and RBM5 are molecular candidates for the diagnosis and treatment of FSGS. However, our results are preliminary, and further work is needed to decipher these candidate genes.

Author contributions

Q.M and Z.H designed the research; Z.H analyzed the data and performed the research; D.Z wrote the manuscript. All authors read and approved the final manuscript.

Qianhong Ma and Dongmei Zhang contributed equally to this work and should be considered co-first authors. 

Funding

This work was supported by grants from the National Natural Science Foundation of China (Grant No. 81200453).

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Supplementary Table 1

gene.symbols

logFC

AveExpr

ts

P.Value

adj.P.Val

B

RPS4Y1

2.569009422

9.570289361

3.101358611

0.004371381

0.011115768

-2.711721859

PLPP3

2.485089122

9.251833831

7.62668606

2.66E-08

7.13E-07

9.068379301

DDX3Y

2.478844353

7.003027727

2.920309189

0.006843408

0.016208722

-3.132862184

SART3

2.470577198

7.660356798

12.25209245

9.40E-13

5.82E-10

19.16340462

TCF4

2.418989646

7.335132517

11.59356528

3.44E-12

1.27E-09

17.89787797

CYP4A11

-2.412274749

9.12866545

-6.123580669

1.33E-06

1.42E-05

5.192258061

FOSB

-2.356258176

8.074381933

-7.877904555

1.42E-08

4.53E-07

9.690625188

TROVE2

2.355365338

9.457713493

7.605950945

2.80E-08

7.39E-07

9.016659035

IQGAP1

2.290645788

8.14538406

9.69432624

1.94E-10

2.19E-08

13.93671972

MBP

2.255967535

7.587944516

12.62989302

4.56E-13

3.91E-10

19.86587003

CALD1

2.192562666

8.074505734

11.18804096

7.85E-12

2.21E-09

17.09171526

EGR1

-2.182777112

10.2801361

-8.747798502

1.72E-09

1.01E-07

11.78050827

RBFOX2

2.164462681

8.69816763

10.33705244

4.72E-11

8.22E-09

15.33081151

IGFBP5

2.148130254

9.995294632

5.537086859

6.45E-06

5.09E-05

3.626766637

CDC42BPA

2.079395261

9.770721553

7.925115034

1.26E-08

4.20E-07

9.806647308

SMAD1

2.076607382

7.949965987

8.465377555

3.38E-09

1.61E-07

11.11324049

G6PC

-2.074536589

6.566342687

-4.459033616

0.000121983

0.000557225

0.736400459

ALB

-2.073497949

9.571928342

-4.114808392

0.000309678

0.001209773

-0.171567409

ZEB1

2.066318697

8.614493251

10.45925661

3.63E-11

7.03E-09

15.5895635

TPM1

2.060897076

9.232448331

10.04753441

8.88E-11

1.30E-08

14.70977983

FERMT2

2.05930113

7.350506265

7.771402732

1.85E-08

5.49E-07

9.427823748

LUC7L3

2.047718235

8.759118544

8.718232863

1.85E-09

1.06E-07

11.71116708

RAN

2.025548667

10.29196832

11.46235083

4.49E-12

1.47E-09

17.63930778

SLC25A36

2.003665861

8.269066322

8.586494779

2.53E-09

1.31E-07

11.40073865

HLA-DRA

1.992802428

11.12961293

7.574703865

3.03E-08

7.84E-07

8.938615772

HTR2B

1.980142562

6.49870054

5.606952454

5.34E-06

4.35E-05

3.814138409

PCYOX1

1.966411418

7.433879894

7.068593108

1.10E-07

2.03E-06

7.65787493

ATRX

1.961414528

6.588331245

11.16381872

8.25E-12

2.27E-09

17.04289981

DYNC1LI2

1.955508721

9.605304116

10.85559765

1.57E-11

3.64E-09

16.41511633

CD99

1.9554319

9.927197771

8.57318363

2.61E-09

1.33E-07

11.36923982

CHD4

1.94490416

8.814477542

16.59170115

5.35E-16

9.51E-12

26.31762235

MYLIP

1.938154662

9.419703765

7.548038454

3.24E-08

8.23E-07

8.871918527

HNRNPF

1.934376435

8.42993207

13.0216741

2.19E-13

2.32E-10

20.57678872

HLA-DQB1

1.933559554

9.283173095

6.877515473

1.81E-07

2.96E-06

7.16657257

FHL1

1.916965148

7.704294928

10.06888924

8.47E-11

1.27E-08

14.75597408

CTSZ

-1.912814074

8.256096924

-9.422794947

3.59E-10

3.49E-08

13.3308037

DDX17

1.900816911

7.156016292

6.849768897

1.94E-07

3.11E-06

7.094896981

THUMPD1

1.891011353

7.141971356

13.73428793

6.01E-14

1.12E-10

21.82576576

PPP3R1

-1.881530742

7.627366273

-7.148865697

8.96E-08

1.74E-06

7.863044486

TNFRSF11B

1.87836792

7.853323079

7.02736233

1.23E-07

2.20E-06

7.552206366

XIST

-1.863860797

7.615665673

-2.956875261

0.006256155

0.015025427

-3.048847061

SON

1.851258175

7.997946847

8.840334385

1.39E-09

8.53E-08

11.99675806

GLUL

1.846739708

7.937765075

9.235752198

5.51E-10

4.67E-08

12.90751377

DKK3

1.838529383

7.235094143

6.88002716

1.80E-07

2.95E-06

7.173056725

BTG1

1.820054032

7.907833291

11.00485807

1.15E-11

2.94E-09

16.72066783

TRIB2

1.81921404

8.182950746

8.768912824

1.64E-09

9.74E-08

11.82995473

RBM5

1.808667325

8.306181873

13.85226391

4.87E-14

1.09E-10

22.02721576

HLA-B

1.803285216

12.34984324

6.340923899

7.42E-07

8.94E-06

5.766672368

MYH10

1.802983311

6.632652763

13.31027341

1.29E-13

1.80E-10

21.08934934

STAG2

1.793115173

5.860191501

13.76494033

5.69E-14

1.12E-10

21.87824888

GBP1

1.783205635

6.97780633

8.494851598

3.15E-09

1.53E-07

11.18338801

RPA1

1.779726763

9.056001662

9.084532378

7.82E-10

5.91E-08

12.56176241

BPTF

1.776426474

7.907083176

7.356751223

5.27E-08

1.17E-06

8.390867161

PCK1

-1.772301574

10.99438749

-4.775458625

5.15E-05

0.000272763

1.580947098

BTN3A3

1.772184439

6.173764331

8.425121851

3.73E-09

1.74E-07

11.01724161

TAGLN

1.764332395

9.007016683

5.779780851

3.34E-06

3.00E-05

4.276795382

PSMA7

1.763068956

6.889044422

11.69687487

2.80E-12

1.22E-09

18.09994223

HLA-DPA1

1.762375082

10.82315093

6.098738113

1.42E-06

1.50E-05

5.126369472

MYLK

1.75861114

10.39524956

6.152944852

1.23E-06

1.34E-05

5.270080435

YIPF6

1.747988074

9.294461389

8.337256865

4.61E-09

2.03E-07

10.80694239

COL3A1

1.741079028

10.03488916

6.880350494

1.80E-07

2.95E-06

7.173891393

ACTB

1.739818254

10.62946301

4.630671948

7.64E-05

0.000379292

1.193646749

DHX15

1.737775319

10.02003843

9.85519139

1.36E-10

1.72E-08

14.29091385

PPIG

1.731145889

7.407036172

7.251707604

6.89E-08

1.43E-06

8.124803038

SCAF11

1.721893208

9.371324456

10.2275776

5.99E-11

9.60E-09

15.09731143

HPGD

-1.717401682

10.07266901

-6.740627536

2.59E-07

3.87E-06

6.812168581

DPP8

1.715249149

9.638588643

8.318534824

4.82E-09

2.09E-07

10.76199707

WSB1

1.714938423

8.596432117

6.91877743

1.62E-07

2.75E-06

7.273007517

ENC1

1.714016384

6.351234133

9.286980973

4.90E-10

4.30E-08

13.02392792

FN1

1.713084538

9.852710944

6.048869862

1.62E-06

1.68E-05

4.993973035

UPF3A

1.706676789

8.950115438

11.59802708

3.41E-12

1.27E-09

17.90663237

KRT19

1.703164941

8.133804492

4.840602977

4.31E-05

0.000235133

1.755562232

BCLAF1

1.697469525

8.139217001

9.830297038

1.44E-10

1.80E-08

14.23633277

FBXO21

1.697287783

9.28818289

5.462538041

7.90E-06

5.94E-05

3.426663378

IFNGR1

1.695558168

9.158556204

8.094628782

8.32E-09

3.11E-07

10.22081389

HCK

1.695111511

7.690905947

7.857688804

1.49E-08

4.67E-07

9.640854784

CXCL2

-1.686811585

6.477878156

-4.50798237

0.000106777

0.000500487

0.866554554

PHACTR2

1.679845963

7.782456361

7.807529456

1.69E-08

5.14E-07

9.517134367

HNRNPM

1.673764753

11.0821542

11.69753948

2.80E-12

1.22E-09

18.10123784

CAMK2N1

-1.651602298

10.19208306

-6.765897326

2.42E-07

3.70E-06

6.877740372

FUS

1.650968802

9.619854977

13.27029455

1.39E-13

1.82E-10

21.01889984

PLPBP

1.650135185

5.59234436

12.81694912

3.21E-13

2.98E-10

20.20749556

PSMB8

1.646543823

10.456135

7.886039857

1.39E-08

4.48E-07

9.71063892

SEC63

1.640469887

7.954060099

6.382735607

6.64E-07

8.19E-06

5.876729688

PALLD

1.635448714

8.943061698

7.556440719

3.17E-08

8.09E-07

8.892944454

ZBTB16

1.635125895

7.66230392

3.653696968

0.001057166

0.003348994

-1.358707431

RBM25

1.63291277

6.324655258

8.650201845

2.17E-09

1.18E-07

11.5511557

TYROBP

1.630051539

7.893243583

5.141218255

1.90E-05

0.00012023

2.563038692

DACH1

1.623091259

7.101855154

6.801703043

2.20E-07

3.44E-06

6.970537686

PSMC3

1.612543512

9.366600505

5.93353245

2.21E-06

2.14E-05

4.68711502

RBMS1

1.6103609

8.650805194

4.65105936

7.23E-05

0.000362333

1.248105923

PGK1

1.607870056

10.36413941

5.403307466

9.28E-06

6.73E-05

3.267574125

NPEPPS

1.606521068

8.421329051

7.835158094

1.58E-08

4.88E-07

9.585322216

PTPRB

1.604906513

9.444331687

7.330988837

5.62E-08

1.23E-06

8.325736785

TNPO1

1.599343907

9.775968964

8.87594367

1.27E-09

8.10E-08

12.07966014

EZR

1.591497713

8.757810465

5.97334531

1.98E-06

1.97E-05

4.793136085

MAGED2

1.586605561

10.30790428

12.89378949

2.78E-13

2.81E-10

20.3466641

ANKRD12

1.580638115

7.392354398

6.693843176

2.92E-07

4.26E-06

6.690596121

FNBP1

1.575997647

8.157390697

8.31499427

4.86E-09

2.10E-07

10.75349206

NR4A2

-1.57481871

6.24079609

-8.527861329

2.91E-09

1.45E-07

11.26180965

SERBP1

1.573844316

8.951883468

11.22792569

7.23E-12

2.19E-09

17.17193154

SPAG9

1.573771613

5.928584131

12.82512531

3.16E-13

2.98E-10

20.22233593

SCAMP1

1.572326984

7.135740239

7.680340099

2.32E-08

6.50E-07

9.201956772

RAB1A

1.571530153

8.448144883

6.477980274

5.16E-07

6.71E-06

6.126856918

TAOK3

1.570396428

7.085559648

9.345798665

4.28E-10

3.85E-08

13.15714066

SRGN

1.568368396

9.423138514

5.18175814

1.70E-05

0.000109899

2.672037194

ZFYVE21

1.5676445

6.846082627

7.262978403

6.69E-08

1.40E-06

8.153414092

HRG

-1.562922635

8.320562127

-4.315974661

0.000179859

0.000770138

0.357348521

PWP1

1.561834997

8.446947472

8.857117309

1.33E-09

8.24E-08

12.03585221

SKAP2

1.561530224

9.277742191

7.33501641

5.57E-08

1.22E-06

8.335924267

UBXN4

1.551349919

9.105993907

6.390043816

6.51E-07

8.06E-06

5.895950838

PECAM1

1.550870521

9.054536986

6.663178499

3.17E-07

4.53E-06

6.610791348

MBNL2

1.550572397

7.066502239

10.39994319

4.12E-11

7.62E-09

15.46422432

SOX9

-1.548564432

8.004071342

-6.562764085

4.13E-07

5.60E-06

6.348811078

TRIM2

1.546572067

8.578770031

8.831695492

1.41E-09

8.68E-08

11.97661948

HNRNPR

1.545721471

9.080452193

10.58685737

2.76E-11

5.70E-09

15.85761598

MYOF

1.54258444

8.617870178

7.043673577

1.18E-07

2.13E-06

7.594032778

NR3C1

1.537314952

8.658438842

7.183782173

8.20E-08

1.62E-06

7.95205499

TFPI2

1.530489929

8.411366859

3.999919431

0.000421606

0.001565513

-0.471022596

TMEM47

1.524753951

8.263583054

6.004266035

1.83E-06

1.84E-05

4.875407299

PURA

1.522929493

7.624254878

6.128405127

1.31E-06

1.41E-05

5.20504847

RAB31

1.519313807

8.597342752

6.411935462

6.15E-07

7.72E-06

5.95349937

CYB5B

1.515075529

7.815221603

11.7624269

2.46E-12

1.16E-09

18.22746762

RIT1

1.514871553

7.235874533

7.407198303

4.63E-08

1.07E-06

8.518169631

PRPF31

1.51015233

6.970023854

7.948526057

1.19E-08

4.05E-07

9.864072564

ALDOB

-1.504568555

8.472159452

-3.061872507

0.004824275

0.012090824

-2.80464753

MAP4

1.503555867

8.031894594

5.975611845

1.97E-06

1.96E-05

4.799168802

RAB2A

-1.502751043

8.315889782

-16.2138848

9.61E-16

9.51E-12

25.7672033

BTN3A2

1.502705438

7.877942556

6.982291597

1.38E-07

2.40E-06

7.436477371

GMDS

1.497770885

9.599429406

7.605970724

2.80E-08

7.39E-07

9.016708396

CD163

1.496518651

7.363641477

3.780358703

0.000756817

0.002538255

-1.036824644

PKN2

1.492252244

7.396561746

9.26254208

5.18E-10

4.46E-08

12.96843728

PICALM

1.48883338

7.452309182

7.882640103

1.40E-08

4.50E-07

9.702276208

LYN

1.48458898

7.748906756

6.995137981

1.33E-07

2.35E-06

7.469486452

ARL6IP5

1.481819394

9.42435093

6.181264534

1.14E-06

1.26E-05

5.345073096

SH3BP5

1.480757339

9.948617496

7.175018224

8.38E-08

1.65E-06

7.929726958

CYTH2

1.478569815

8.695293012

14.0977443

3.17E-14

7.84E-11

22.44167418

HSP90AA1

1.475101446

10.7822491

5.488461362

7.36E-06

5.62E-05

3.49626449

PDPK1

1.474624621

8.188917333

5.243240876

1.44E-05

9.60E-05

2.837346192

SMC3

1.470872089

7.133595994

11.12401077

8.96E-12

2.41E-09

16.96251038

MBTPS1

1.465393655

8.496997806

7.766899015

1.87E-08

5.53E-07

9.416678054

TIMP3

1.463150322

9.290336056

7.335248955

5.56E-08

1.22E-06

8.336512413

SSRP1

1.462015221

7.615804716

9.043612741

8.60E-10

6.37E-08

12.46766016

NAA35

1.458486577

6.726259229

8.092465915

8.37E-09

3.11E-07

10.21555345

KIF5B

1.454182588

8.394875765

5.616323045

5.20E-06

4.27E-05

3.839255868

DHRS7

1.454116053

7.744616405

9.572938924

2.56E-10

2.65E-08

13.66709981

CNPY2

1.453764841

8.534477403

8.634059418

2.26E-09

1.20E-07

11.51309488

FKBP5

1.452723718

7.291698701

4.149986734

0.000281682

0.001116854

-0.079470163

COL6A3

1.447501816

7.827000817

4.905985588

3.61E-05

0.000203504

1.930987853

COL4A2

1.446665268

7.456526747

6.876736022

1.81E-07

2.96E-06

7.164560203

CALB1

-1.44533189

9.757638848

-2.605850418

0.01452899

0.030801016

-3.831208449

IGF1

-1.437115619

9.406113438

-3.336824653

0.002407335

0.006723822

-2.146238464

NELFCD

1.434489749

9.91051469

11.67139035

2.95E-12

1.22E-09

18.0502204

YWHAB

1.431615777

8.640142735

5.544355542

6.32E-06

5.00E-05

3.646268215

RGCC

1.429769752

7.679603868

4.732902335

5.78E-05

0.000299666

1.466986783

SLC25A6

1.428984166

11.61631244

9.101576376

7.52E-10

5.75E-08

12.60089001

ID3

1.428233521

10.14589451

8.266105018

5.47E-09

2.29E-07

10.63587854

TRA2B

1.424948669

9.497698712

8.624014607

2.31E-09

1.22E-07

11.48939306

GDI2

1.424531564

9.161869349

6.615965347

3.59E-07

4.97E-06

6.48773575

CRHBP

1.423223574

10.85750131

3.501852173

0.001572242

0.004679346

-1.739506163

EID1

1.422600712

9.21370437

7.376173329

5.01E-08

1.13E-06

8.439915301

SET

1.420718447

10.19887727

8.55531067

2.73E-09

1.37E-07

11.32690802

FOS

-1.420440832

9.273302043

-3.000916158

0.005612259

0.013756238

-2.946942453

VDAC1

1.415009019

8.172894871

7.118018567

9.71E-08

1.85E-06

7.784289799

WASHC4

1.412990786

7.331446343

8.01490027

1.01E-08

3.56E-07

10.02649009

UMOD

-1.412526572

11.37016849

-2.38566243

0.024069305

0.047104894

-4.291378203

SERTAD2

1.411604795

8.175798374

8.513812449

3.01E-09

1.48E-07

11.22845173

PQBP1

1.411256434

7.748400335

6.769612323

2.40E-07

3.68E-06

6.887374717

CEP350

1.409862497

6.337432566

7.135793044

9.27E-08

1.79E-06

7.829682626

GLYAT

-1.407015938

9.579743485

-4.089844356

0.000331191

0.001276585

-0.236812106

ICAM2

1.406910331

9.771163793

5.247150209

1.42E-05

9.52E-05

2.84785686

GNB1

1.402945544

9.130430125

10.649444

2.42E-11

5.13E-09

15.98830239

HLA-DMB

1.401194865

10.17298113

8.848947598

1.36E-09

8.38E-08

12.01682652

EDNRB

1.398466757

8.373823696

4.94196731

3.27E-05

0.000188026

2.02759004

ESF1

1.398047621

7.471743775

13.05457765

2.06E-13

2.30E-10

20.63569798

AIDA

1.397041007

7.692499594

8.873300307

1.28E-09

8.11E-08

12.07351212

TOP1

1.396150489

8.310821776

7.122657614

9.59E-08

1.83E-06

7.796140615

SNX1

1.393315129

7.77005298

9.617845685

2.31E-10

2.46E-08

13.76708049

CLDND1

1.389278478

8.356235731

7.353059136

5.32E-08

1.18E-06

8.381538064

TSPAN3

1.38922393

9.719295375

4.512799487

0.000105387

0.000495607

0.879374553

SLC7A8

1.388681022

9.335582412

4.354999431

0.000161804

0.000706402

0.460535324

ARHGAP5

-1.388500976

8.373041869

-8.710601093

1.88E-09

1.07E-07

11.69324854

ILF3

1.386329503

6.940492403

8.230768777

5.97E-09

2.45E-07

10.55066888

ATG12

1.384776561

7.242819619

7.607340868

2.79E-08

7.39E-07

9.020127669

ZNF148

1.382618168

8.099582442

12.45022932

6.42E-13

4.62E-10

19.53391159

DLC1

1.379754481

9.408529743

8.406044121

3.90E-09

1.80E-07

10.97166939

MYDGF

1.379051924

8.854924998

9.767015768

1.65E-10

1.97E-08

14.09720669

PSD3

1.379024578

6.766600545

5.210698818

1.57E-05

0.000103008

2.749850991

CDC37

1.377078992

9.468182931

10.45472383

3.67E-11

7.04E-09

15.58000157

KPNB1

1.376929789

9.458847188

9.024827414

8.99E-10

6.56E-08

12.4243824

FOXN3

1.375967793

6.83901797

8.165674647

6.99E-09

2.76E-07

10.39326148

LIMCH1

1.375409137

9.097684708

5.56555133

5.97E-06

4.77E-05

3.703125677

MLEC

1.374524107

9.243909884

6.009168559

1.80E-06

1.82E-05

4.888445732

FABP5

1.370563815

7.473351905

3.638781888

0.001099405

0.003461152

-1.396367326

SCNN1A

-1.367945371

9.167955994

-4.114464255

0.000309965

0.001210427

-0.172467464

TPR

1.367879082

8.216314258

4.77899159

5.10E-05

0.000270724

1.590412014

HSP90AB1

1.36640902

9.19132412

5.419142441

8.89E-06

6.51E-05

3.310113519

ABCF2

1.364898619

7.12749747

8.687031396

1.99E-09

1.11E-07

11.63785903

HNRNPD

1.36405605

9.094619756

8.738208165

1.76E-09

1.02E-07

11.75802893

TGFBR2

1.362070619

7.153715484

5.381838143

9.84E-06

7.03E-05

3.209890462

CD14

1.361767811

8.618764289

5.636868061

4.92E-06

4.09E-05

3.894313936

FRZB

1.361170602

9.126704666

5.082735903

2.22E-05

0.000137296

2.405816869

BBS4

1.360150632

7.202996413

8.971308938

1.02E-09

7.08E-08

12.30081903

HLA-G

1.359808391

10.72098347

5.244182836

1.43E-05

9.58E-05

2.839878762

SWAP70

1.359427598

9.113593244

5.306026158

1.21E-05

8.33E-05

3.006138059

TNPO3

1.358231729

7.693616767

10.42872351

3.88E-11

7.26E-09

15.52510066

LACTB2

-1.355852383

7.530616

-6.671617676

3.10E-07

4.45E-06

6.632763722

CEP57

1.352993373

5.052119046

12.37324173

7.44E-13

4.88E-10

19.39050197

MYO6

1.352421261

9.176047271

5.255177235

1.39E-05

9.36E-05

2.869438127

EPRS

1.351852256

8.607022753

8.649814499

2.17E-09

1.18E-07

11.55024283

LRBA

-1.349799955

8.811192382

-8.795602403

1.54E-09

9.30E-08

11.8923698

CXCR4

1.347649061

8.222517485

3.684201964

0.000975644

0.003135087

-1.281519314

SRRM2

1.347568684

8.460042382

5.001654871

2.78E-05

0.000165066

2.187913815

RPLP0

1.34749233

11.59881981

12.16742107

1.11E-12

6.17E-10

19.00364445

ITGA8

1.343209501

8.187995584

5.506346749

7.01E-06

5.43E-05

3.544273858

DDX39A

1.342371076

8.373749992

14.91487814

7.84E-15

2.61E-11

23.77683811

SSBP2

1.341694376

8.032258834

10.36379012

4.46E-11

8.01E-09

15.38759602

HMGCR

1.340708611

7.991412194

7.949010066

1.19E-08

4.05E-07

9.865259037

STX11

1.338253755

6.131744045

8.963225837

1.04E-09

7.12E-08

12.28212241

TTF1

1.335503511

7.454129952

7.565990872

3.10E-08

7.95E-07

8.916832104

AZIN1

1.331515063

6.892036517

13.60307467

7.59E-14

1.17E-10

21.59996308

OAS1

1.328744906

7.180037476

4.581369291

8.74E-05

0.000424389

1.062067683

PLEKHB1

-1.325016546

7.570574431

-8.601436826

2.44E-09

1.27E-07

11.43606785

TMEM230

1.322637972

8.978392691

6.664841119

3.15E-07

4.52E-06

6.615120736

BMPR2

1.321446254

6.459157792

7.530440342

3.39E-08

8.48E-07

8.827852131

ZNF207

1.320509797

9.748881671

7.924554461

1.27E-08

4.20E-07

9.805271384

RAD21

1.320165778

7.728846175

7.217996731

7.51E-08

1.53E-06

8.039137581

HCLS1

1.318686529

9.088478783

7.557331729

3.17E-08

8.09E-07

8.895173607

COG7

1.318315987

7.957007308

6.947663903

1.51E-07

2.60E-06

7.34740937

TSPAN5

1.317168741

7.574432985

7.172334408

8.44E-08

1.66E-06

7.922887565

CALR

-1.316887257

9.46952319

-6.187797543

1.12E-06

1.24E-05

5.362364282

CASP1

1.315406814

7.280061706

6.917554285

1.63E-07

2.75E-06

7.269855086

CD53

1.314626339

9.310209635

4.82374315

4.51E-05

0.000244358

1.710352793

STAT1

1.311254404

7.989702392

8.164628307

7.01E-09

2.76E-07

10.39072663

NASP

1.311023485

8.658422087

8.195333579

6.50E-09

2.59E-07

10.46505182

ATF3

-1.309777911

9.32422844

-4.436888056

0.000129551

0.000586505

0.677587657

LRRFIP1

1.308595239

9.842768068

6.02460185

1.73E-06

1.77E-05

4.929480606

BLVRA

1.307890164

7.463070636

7.883905699

1.40E-08

4.49E-07

9.705389497

TRAM2

1.307573251

8.603172154

6.048923045

1.62E-06

1.68E-05

4.994114324

SPTLC1

1.306960571

7.365444445

8.208877143

6.29E-09

2.54E-07

10.49779515

COL4A3

1.303589773

10.63769098

5.004570724

2.75E-05

0.000164118

2.195748058

DDX41

1.303536766

9.473571199

11.00669283

1.14E-11

2.94E-09

16.72440575

ACTR3

1.302268329

10.75060111

7.097878642

1.02E-07

1.93E-06

7.732811998

ACLY

1.302204054

8.910503391

7.337433307

5.53E-08

1.22E-06

8.342036709

FNTA

1.301806985

9.235960273

8.985880851

9.85E-10

6.90E-08

12.33450181

FCN1

1.301737217

7.825729707

4.507676388

0.000106866

0.000500799

0.865740298

GPM6B

1.30102056

7.554873441

5.23789948

1.46E-05

9.70E-05

2.822985149

NUDC

1.299825767

10.1129032

9.124297995

7.13E-10

5.57E-08

12.65298921

PHB

1.299280352

9.036698753

8.34467426

4.52E-09

2.00E-07

10.82473593

USP1

1.299122208

5.712328518

6.903628133

1.69E-07

2.81E-06

7.233951504

UGCG

1.299100428

7.637255196

6.564098659

4.11E-07

5.59E-06

6.352299401

NUP88

1.298049189

7.778731314

11.63242519

3.19E-12

1.26E-09

17.97404068

LEPROT

1.297113978

11.60207857

4.961828441

3.10E-05

0.000180137

2.080928206

SORD

-1.296373291

9.712182519

-3.408042698

0.002004522

0.00576643

-1.971684138

RNASEH1

1.296211957

8.062974187

7.743815272

1.98E-08

5.73E-07

9.359509858

BGN

-1.293749031

10.83426956

-5.497336355

7.19E-06

5.53E-05

3.520088593

EPB41L1

-1.293565904

8.131970301

-8.910275853

1.18E-09

7.65E-08

12.15942346

NT5DC2

1.290854598

7.006657669

5.49509333

7.23E-06

5.55E-05

3.514067608

EIF5

1.290509517

9.992535565

5.777169499

3.37E-06

3.01E-05

4.26981525

JUND

-1.288819693

11.17357526

-13.61921949

7.38E-14

1.17E-10

21.62784642

PLK2

1.287415552

7.339410911

5.759288171

3.53E-06

3.14E-05

4.222009283

M6PR

1.286522924

7.51082701

7.771500991

1.85E-08

5.49E-07

9.428066888

DHRS7B

1.286003609

9.123234779

8.514469828

3.01E-09

1.48E-07

11.23001323

TCF7L1

-1.284417857

9.471134171

-6.21840816

1.03E-06

1.16E-05

5.443338468

TMEM140

1.284344829

9.254354193

10.94233816

1.31E-11

3.20E-09

16.59303621

CYP27B1

-1.28385958

8.032205684

-5.997251595

1.86E-06

1.87E-05

4.856749376

KRAS

1.281495706

8.377790663

6.362344392

7.01E-07

8.55E-06

5.823074564

GMFG

1.279945687

9.288317601

7.459463711

4.06E-08

9.68E-07

8.64973168

PSMA3

1.279565875

10.16490647

5.83933803

2.85E-06

2.64E-05

4.435894253

CNOT2

1.279300816

8.782442522

6.434254514

5.79E-07

7.35E-06

6.01212757

KLC1

1.277054189

9.104980272

7.524681574

3.44E-08

8.56E-07

8.813423521

PLPPR1

-1.276165848

8.240622771

-3.816088742

0.000688405

0.002344447

-0.945391009

ABCG1

1.275777921

7.186008684

6.811441439

2.15E-07

3.37E-06

6.995753313

DAZAP2

1.274142925

9.739018005

7.637559175

2.59E-08

7.01E-07

9.095478672

ERLIN2

-1.273846883

9.305200067

-7.370850264

5.08E-08

1.14E-06

8.426477123

GC

-1.272445074

6.332951466

-2.524545429

0.017546408

0.036095513

-4.004098064

RNF13

1.268534955

10.26760703

5.355043954

1.06E-05

7.48E-05

3.137888261

COL1A2

1.266974585

7.179573515

3.501641194

0.001573104

0.004681133

-1.740031084

ZC3H15

1.266150743

9.172149178

5.200299793

1.61E-05

0.000105414

2.721890801

KMT5B

1.265913904

8.649285188

7.46494521

4.00E-08

9.61E-07

8.663510146

PUM1

1.263073454

8.134411951

6.955641137

1.48E-07

2.55E-06

7.367939869

ADD3

1.258422615

8.997988364

5.947988367

2.12E-06

2.08E-05

4.725622462

KPNA2

1.255126625

8.907075287

6.720095976

2.73E-07

4.03E-06

6.758843409

HLA-DMA

1.254424027

10.37397051

5.785073702

3.29E-06

2.96E-05

4.290942076

THEMIS2

1.253798071

7.019422688

6.789113515

2.28E-07

3.53E-06

6.937924834

GNL3L

1.252325393

7.124510447

10.81838227

1.69E-11

3.89E-09

16.33847971

PSMB4

1.249957625

10.72159802

9.265970538

5.14E-10

4.44E-08

12.97622687

AGMAT

-1.248270761

10.8536785

-3.824270959

0.000673611

0.002304987

-0.924415444

GIPC2

-1.247317538

7.723359098

-4.309932572

0.000182827

0.000781047

0.341387838

DHX9

1.246849962

6.351974134

7.128339548

9.45E-08

1.81E-06

7.810652181

EWSR1

1.246648172

8.535866582

10.08327007

8.21E-11

1.24E-08

14.78704752

SETD5

1.245898909

7.482287092

7.89652774

1.36E-08

4.40E-07

9.736427478

EIF4G1

1.244109972

7.486954023

8.936556557

1.10E-09

7.39E-08

12.22037107

SRSF3

1.243986139

9.803111657

7.286475154

6.30E-08

1.34E-06

8.21301214

ILF2

1.243442066

9.552958146

6.68055437

3.03E-07

4.37E-06

6.656023645

CYP26B1

-1.242450416

8.634347541

-2.397548309

0.02343442

0.046015965

-4.26720407

SERPINE1

1.240736407

6.725458286

4.521128924

0.000103025

0.000486376

0.901546668

PSMD4

1.240423225

10.38557871

10.02544818

9.32E-11

1.33E-08

14.66193831

PDLIM3

1.239425245

7.796445864

8.153301921

7.21E-09

2.81E-07

10.36327807

TMEM87A

1.238174918

8.636838914

8.683893986

2.01E-09

1.12E-07

11.63048026

PRRC2C

1.238003417

7.756011399

7.200125227

7.86E-08

1.58E-06

7.993668362

SNX4

1.237495753

7.321680798

7.530106662

3.39E-08

8.48E-07

8.827016208

NRP1

1.236313178

9.129569229

5.883209216

2.53E-06

2.39E-05

4.552965336

WAC

1.235001654

7.03036538

7.197894999

7.90E-08

1.58E-06

7.98799151

SASH1

1.234739955

8.294239034

7.261823684

6.71E-08

1.40E-06

8.15048352

TAP1

1.234734308

9.251835212

8.342764658

4.55E-09

2.01E-07

10.82015571

CHMP2A

1.234514701

10.38085677

5.726260811

3.86E-06

3.38E-05

4.133668805

ARPC1B

1.23312388

9.847594921

6.629826451

3.46E-07

4.86E-06

6.523885973

C1QA

1.232874166

7.267276992

3.49242546

0.001611225

0.004776231

-1.762948256

ZFPM2

-1.231930712

8.037949772

-5.308203625

1.20E-05

8.29E-05

3.011991311

APLP2

1.231910612

10.32603877

7.606109272

2.80E-08

7.39E-07

9.017054163

UTP14A

1.231347458

7.661719061

7.121605911

9.62E-08

1.84E-06

7.793454173

CAT

1.229093195

8.87973752

6.872999304

1.83E-07

2.99E-06

7.15491194

TIA1

1.227747542

7.247584903

9.010951208

9.29E-10

6.61E-08

12.39238301

NAGK

1.22754899

9.926020105

10.36793433

4.42E-11

8.00E-09

15.39638877

TM9SF1

1.227465595

8.623773028

8.253859905

5.64E-09

2.34E-07

10.60636978

GLG1

1.226621678

9.826971817

5.632513248

4.98E-06

4.12E-05

3.882644978

XPNPEP1

1.225389014

8.466825777

7.86771117

1.46E-08

4.60E-07

9.665536137

HYAL2

1.22355501

9.281267332

6.500981316

4.86E-07

6.40E-06

6.18713754

ASF1A

1.223294692

7.739507727

7.51950823

3.48E-08

8.66E-07

8.80045816

WARS

1.221822477

9.608411649

7.31487298

5.86E-08

1.27E-06

8.284953181

TMCO3

1.22011721

9.102616418

4.979257633

2.95E-05

0.000173536

2.127743623

KTN1

1.218156632

6.898784102

5.40406927

9.26E-06

6.72E-05

3.269620769

NNT

1.215568091

6.952880722

5.386246015

9.72E-06

6.96E-05

3.221734225

XPNPEP2

-1.215559549

8.851730714

-3.325159474

0.002480356

0.006893212

-2.174682385

FLRT3

1.210792751

10.14839181

4.168211768

0.000268176

0.001070866

-0.031686858

FADS3

1.209980177

7.375563699

8.103163063

8.15E-09

3.06E-07

10.24156454

ENO1

1.209953356

7.883385651

6.223548255

1.01E-06

1.15E-05

5.45692833

HLA-F

1.209909743

11.83477086

8.124091232

7.74E-09

2.96E-07

10.29240912

PTPRC

1.208351307

5.832030673

8.047207456

9.35E-09

3.38E-07

10.10533478

SYT1

-1.208255658

7.30889895

-4.705882758

6.23E-05

0.000319624

1.394681992

COMT

1.206977299

9.312304131

7.752820425

1.94E-08

5.66E-07

9.381819803

PLS1

-1.206303455

8.261543055

-5.496977729

7.19E-06

5.53E-05

3.519125937

ITGB2

1.205909695

7.91890843

5.254326121

1.39E-05

9.37E-05

2.867149862

SMARCE1

1.205666484

8.972747998

7.373668132

5.04E-08

1.13E-06

8.433591315

SEC14L1

1.205033056

7.939442936

6.08339441

1.48E-06

1.55E-05

5.085651874

POSTN

1.201856865

10.82658185

3.379886179

0.00215532

0.006130585

-2.040877409

DEFB1

-1.200644494

10.99447884

-3.02989907

0.005223435

0.012932645

-2.879463619

TYRP1

-1.200351272

6.207994431

-3.539545898

0.001425307

0.004302374

-1.645531253

IMP3

1.199878035

9.403506224

8.37029393

4.25E-09

1.92E-07

10.88613742

SMARCA2

1.199515299

6.947329006

7.267526219

6.61E-08

1.38E-06

8.164954491

AKAP8L

1.199316813

6.916908369

7.807966077

1.69E-08

5.14E-07

9.518212724

PSMD13

1.198610121

8.052040062

10.25115059

5.69E-11

9.46E-09

15.14772671

COPS8

1.1970076

9.204275007

7.044000749

1.17E-07

2.13E-06

7.594871427

AKAP13

1.19666266

7.792140231

6.987365814

1.36E-07

2.38E-06

7.449517889

RRAD

1.196066763

6.953293655

7.077275531

1.08E-07

2.01E-06

7.680102108

PLEKHO1

1.195459638

7.723639306

7.159751859

8.72E-08

1.70E-06

7.89081119

FOLR1

-1.193436488

6.956916716

-3.734922458

0.000853477

0.002814152

-1.152704059

PPIC

1.192607632

8.477886659

6.960597292

1.46E-07

2.53E-06

7.380691668

SNX2

1.192127734

8.610500524

6.157301546

1.21E-06

1.33E-05

5.281621255

MBD4

1.185770843

8.009202546

7.069175367

1.10E-07

2.03E-06

7.659365794

AP3D1

1.18500723

8.6135603

7.725903422

2.07E-08

5.94E-07

9.315102851

HBB

1.184947119

11.93377932

3.343143234

0.002368653

0.006631573

-2.130813886

RNF114

1.183206283

9.30418479

9.465288756

3.26E-10

3.26E-08

13.42629734

ABHD17A

1.181106471

9.170196843

8.329715797

4.69E-09

2.06E-07

10.78884453

LRRC42

1.180209467

7.573503367

8.941836312

1.09E-09

7.34E-08

12.23260386

DDAH1

-1.180008114

11.46433716

-5.151767722

1.84E-05

0.000117466

2.591402158

TSC22D3

1.179816234

8.262843013

4.224652868

0.000230275

0.000942027

0.116582968

GYG1

1.177529499

9.834468492

6.016373152

1.77E-06

1.80E-05

4.907603688

UQCRC2

1.176924599

9.899057439

7.23706376

7.15E-08

1.47E-06

8.087606913

FKBP11

1.176919235

8.727123226

6.266120256

9.06E-07

1.04E-05

5.569402926

TM4SF1

1.176815697

8.331708401

5.239153764

1.45E-05

9.68E-05

2.826357468

AHCYL1

1.176691914

8.745386211

5.330391797

1.13E-05

7.91E-05

3.071632149

SLC28A1

-1.176464801

6.772070338

-8.212773139

6.23E-09

2.52E-07

10.50720966

LGALS3

1.176104581

9.941755013

7.549802161

3.23E-08

8.21E-07

8.876332784

CAVIN2

1.174754505

6.982038785

5.495846891

7.22E-06

5.54E-05

3.516090419

SERPINH1

1.174275681

9.648430496

6.407521754

6.22E-07

7.79E-06

5.941900078

ACTA2

1.173648029

10.38204081

5.932733912

2.21E-06

2.15E-05

4.684987509

HNRNPA2B1

1.173592582

11.06475641

9.665793594

2.07E-10

2.28E-08

13.87352619

AP1S2

1.172535127

8.523906337

5.252624432

1.40E-05

9.41E-05

2.862574763

PNN

1.17087381

7.970795555

7.747188801

1.97E-08

5.70E-07

9.367868879

HLA-C

1.169664398

10.16761106

7.595608208

2.88E-08

7.54E-07

8.990840477

PRPF4B

1.169551009

6.935475927

5.67669604

4.42E-06

3.75E-05

4.000998961

USP48

-1.168630772

9.038096545

-7.747244509

1.97E-08

5.70E-07

9.368006903

MCM7

1.168505141

8.351995021

8.050385686

9.28E-09

3.36E-07

10.11308366

TUG1

1.168335892

9.165666062

6.054292701

1.60E-06

1.66E-05

5.008378733

CASP4

1.167446728

7.679803702

10.35532363

4.54E-11

8.03E-09

15.36962558

FOXO3

1.167411973

8.567526997

5.725633219

3.87E-06

3.38E-05

4.131989636

VAMP2

-1.166211897

7.442364851

-7.873871112

1.43E-08

4.56E-07

9.680699271

PDIA6

1.165260403

10.67248004

5.899377478

2.42E-06

2.31E-05

4.596082594

DDOST

1.164865434

10.9394614

6.470185623

5.27E-07

6.83E-06

6.10641779

PYCARD

1.163566225

7.46929959

5.864762597

2.66E-06

2.50E-05

4.503753551

ZNF24

1.162861739

7.210438195

7.357297992

5.26E-08

1.17E-06

8.392248586

TYRO3

1.161757961

8.189575049

5.5953718

5.51E-06

4.46E-05

3.783092394

ADH6

-1.16166635

8.207644366

-3.436207702

0.001863907

0.005412229

-1.902237878

ACSL3

1.16124775

8.408959315

3.546281515

0.001400494

0.004237233

-1.628698861

PARP2

1.161143625

8.581427075

11.19875359

7.68E-12

2.21E-09

17.11328056

MCCC2

-1.160816294

10.45719644

-5.897428416

2.43E-06

2.32E-05

4.590885687

ZNF22

1.159131186

7.261181636

12.5812987

5.00E-13

4.05E-10

19.77645675

RALBP1

1.158724753

8.274230454

7.859219723

1.49E-08

4.66E-07

9.644625716

NUP85

1.156176785

8.422135666

9.538080515

2.77E-10

2.81E-08

13.5892997

CPD

1.156112532

6.819716927

8.154881627

7.18E-09

2.81E-07

10.36710738

HCP5

1.155541743

8.967916149

5.892526241

2.47E-06

2.34E-05

4.577813709

AGTR1

1.155149645

6.968562585

5.121576314

2.00E-05

0.000125603

2.510230811

LIN37

1.155105526

6.577595527

9.667828073

2.06E-10

2.28E-08

13.87803581

CCT6A

1.154913313

9.14834035

7.235166054

7.18E-08

1.48E-06

8.08278478

HNRNPC

1.154717025

8.873047157

6.813932366

2.13E-07

3.35E-06

7.002201462

SLC13A1

-1.15376563

7.5476979

-3.18681201

0.003526296

0.009294589

-2.508681404

VAMP3

1.152730555

7.405947549

4.957816762

3.13E-05

0.000181512

2.070153776

MAP4K3

-1.150486671

7.339441604

-6.152728191

1.23E-06

1.34E-05

5.269506464

JMJD6

1.150291091

7.191119689

12.55420458

5.27E-13

4.05E-10

19.726484

HLA-DPB1

1.150218342

11.36426215

5.525706581

6.65E-06

5.21E-05

3.596230432

SRRM1

-1.149363305

9.137180405

-5.391189648

9.59E-06

6.88E-05

3.235017125

RRAGC

1.149352733

8.837854366

6.475336686

5.20E-07

6.75E-06

6.119925528

ZNF804A

-1.149172983

8.504688414

-5.863390141

2.67E-06

2.50E-05

4.500091338

PSME3

-1.148471143

9.262437167

-5.79437545

3.21E-06

2.90E-05

4.315800219

RBP4

-1.148117964

8.285701455

-2.430483745

0.021754392

0.043227525

-4.199808903

NMD3

1.147678273

7.096738478

5.993365837

1.88E-06

1.89E-05

4.846412134

PSMB9

1.144615141

10.39353049

6.638465727

3.38E-07

4.76E-06

6.546407914

SYNCRIP

1.144133474

7.533673599

5.254346869

1.39E-05

9.37E-05

2.867205645

YWHAH

1.142268582

8.609758108

3.448365406

0.001806203

0.005267323

-1.872190252

DNAJC8

1.141702145

9.890908337

12.41268851

6.90E-13

4.67E-10

19.46406954

ARF4

1.141135381

9.861513946

4.629461092

7.67E-05

0.000380207

1.190413168

CYP1B1

1.139251767

8.983971841

6.038927934

1.66E-06

1.71E-05

4.967557162

FGL2

1.139176467

10.00389177

5.396769049

9.45E-06

6.81E-05

3.250007688

TAX1BP1

1.137965

10.06609394

7.996955328

1.06E-08

3.69E-07

9.982636271

DNAJC7

1.136922569

8.975904344

5.350564296

1.07E-05

7.55E-05

3.125849226

SNRPB

1.13575687

10.70918168

8.092302578

8.37E-09

3.11E-07

10.21515617

WT1

1.132916354

9.05457011

4.948629513

3.21E-05

0.000185305

2.045480572

SLC13A3

-1.132662093

10.63519462

-3.356064493

0.002291407

0.006445268

-2.099233019

EFNB2

1.132430918

8.078269009

5.104816376

2.09E-05

0.000130601

2.465173592

GATM

-1.132347483

9.378659276

-4.820073926

4.56E-05

0.000246052

1.700515384

CNIH4

1.131683601

8.479595575

5.273080806

1.32E-05

8.97E-05

2.917571743

EPHX1

-1.131399427

8.499935775

-2.800395449

0.00915647

0.020779471

-3.404434478

SLC12A1

-1.131269099

9.982695474

-3.262062692

0.002913818

0.007915227

-2.327789665

DDX18

1.12754023

7.44385403

6.968940574

1.43E-07

2.48E-06

7.402152136

ALAS1

1.125667398

9.259205057

10.4312198

3.86E-11

7.26E-09

15.5303756

PIPOX

-1.123197227

9.810383723

-3.323828884

0.002488819

0.006915008

-2.177924155

TMEM204

1.123075986

10.94367823

5.734623996

3.78E-06

3.30E-05

4.156043341

IFI16

1.121716104

6.377629496

6.221519431

1.02E-06

1.15E-05

5.451564585

FDPS

1.121519611

9.476641456

5.462769647

7.89E-06

5.94E-05

3.427285292

ADSL

1.120968528

9.354870139

11.7975847

2.29E-12

1.13E-09

18.29564463

C21orf59

1.120241495

9.349721825

8.079662341

8.63E-09

3.19E-07

10.18440035

PLXDC2

1.119961822

6.674408432

6.694948461

2.91E-07

4.25E-06

6.693470854

IK

1.119516839

11.08344752

11.17279646

8.10E-12

2.26E-09

17.06100157

EPB41L5

1.119432184

8.719882514

5.112636291

2.05E-05

0.000128284

2.486196259

PAPOLA

1.11940591

9.203346945

6.556215488

4.20E-07

5.67E-06

6.331691821

BABAM1

1.118621088

9.799168898

13.13465146

1.78E-13

2.09E-10

20.77854969

EIF3A

1.118546997

8.851059563

7.373548512

5.05E-08

1.13E-06

8.433289332

10-Sep

1.11477609

7.162778757

5.1308438

1.95E-05

0.000123054

2.535146373

METTL3

1.114576495

9.512185519

10.75712554

1.93E-11

4.32E-09

16.21193997

RYBP

1.114362488

8.074681305

5.558649075

6.08E-06

4.84E-05

3.684612117

SRSF7

1.114068517

7.812810523

6.78434274

2.31E-07

3.57E-06

6.925561917

SPCS2

1.113642182

10.67916436

10.02665475

9.29E-11

1.33E-08

14.66455361

IL6ST

1.112439922

7.249378474

5.2191725

1.53E-05

0.000101131

2.772634323

CASP3

1.112233366

7.060212477

6.02808801

1.71E-06

1.75E-05

4.938747575

SLC22A8

-1.111645351

8.172587127

-2.906495713

0.007078547

0.016675257

-3.164457325

SLC34A1

-1.111436435

8.827172212

-3.462040019

0.001743365

0.005105454

-1.838343558

SLCO3A1

1.110078679

7.643092094

5.674934307

4.44E-06

3.76E-05

3.996281323

H2AFZ

1.109787786

10.36972324

7.309395678

5.94E-08

1.29E-06

8.271084918

TRAM1

1.109674164

9.330891829

5.475848882

7.62E-06

5.78E-05

3.462403688

CPM

1.108350817

6.344952054

4.511146811

0.000105862

0.000497192

0.874975993

SF3B4

-1.106855958

8.447412686

-11.66922686

2.96E-12

1.22E-09

18.04599558

GPKOW

1.105821203

9.402386711

15.05894645

6.17E-15

2.61E-11

24.00537577

COMMD4

1.105582864

8.633374558

9.573721267

2.55E-10

2.65E-08

13.66884399

NKTR

1.105103578

7.436472493

6.31627032

7.93E-07

9.41E-06

5.701708637

GZMB

1.104615964

6.213304274

4.943201366

3.26E-05

0.000187539

2.030903852

PRKAR1A

1.103349619

9.085496498

6.930113803

1.58E-07

2.69E-06

7.302217123

GAR1

1.103322681

8.867905942

11.69566488

2.81E-12

1.22E-09

18.09758328

PCMT1

1.10293129

9.090122013

8.57858399

2.58E-09

1.32E-07

11.38202189

OSBPL1A

1.101591227

7.294578344

6.029552935

1.71E-06

1.75E-05

4.942641418

CSNK1A1

1.101429726

9.136865726

8.271107725

5.41E-09

2.27E-07

10.64792844

FRY

1.101325533

7.416761028

4.840073415

4.32E-05

0.000235359

1.75414204

ETS2

1.10079836

9.955899787

6.80950633

2.16E-07

3.38E-06

6.990743531

C1QBP

1.099436575

9.443174021

7.90432057

1.33E-08

4.35E-07

9.755579866

CAMK1

1.098714967

7.277320139

8.659802769

2.12E-09

1.16E-07

11.57377593

TIGAR

1.098198376

7.2916035

5.49588839

7.21E-06

5.54E-05

3.516201816

ELF1

1.097652061

9.011134077

8.423523203

3.74E-09

1.74E-07

11.01342471

PPFIBP1

1.096172641

6.181551302

6.404621461

6.27E-07

7.83E-06

5.934277118

TCF25

1.096054149

8.091775108

6.227690486

1.00E-06

1.13E-05

5.467878422

CDC27

1.095817747

8.505153326

4.91940844

3.48E-05

0.000197882

1.967020368

SLA

1.094863831

8.203268999

6.487175988

5.04E-07

6.58E-06

6.150962717

NEU1

1.093178847

8.947170302

7.96855995

1.13E-08

3.91E-07

9.913156724

MTDH

1.092673248

9.333952516

4.191473779

0.000251861

0.001016156

0.029369908

RALYL

-1.091884713

8.179522241

-3.770225925

0.0007774

0.002597903

-1.062705647

EGR2

-1.090102058

6.469861827

-4.641895549

7.41E-05

0.000369589

1.223623989

SELENOW

1.089407334

10.83413383

8.564446583

2.67E-09

1.35E-07

11.34855174

DHX35

1.088913326

7.875257997

8.366012273

4.30E-09

1.94E-07

10.87588196

H2AFY

1.088300954

8.945648275

7.534738013

3.35E-08

8.41E-07

8.838617254

FEZ2

1.088132355

9.829802339

6.601202262

3.73E-07

5.13E-06

6.449212407

CXCL1

-1.08797836

7.890182363

-3.583978395

0.001269183

0.003906784

-1.534276712

TUBG1

1.086695059

8.745692451

9.151344631

6.70E-10

5.33E-08

12.71491244

EGFR

1.086214902

6.754578363

6.745928219

2.55E-07

3.84E-06

6.82592863

DERL1

1.086001096

6.89012709

6.279613636

8.74E-07

1.01E-05

5.605021567

CAV1

1.084584181

7.441861966

7.66312002

2.43E-08

6.70E-07

9.159125496

IDH3G

1.082946039

9.572242915

4.996077021

2.82E-05

0.000166977

2.172927883

DDIT4

1.082694616

9.431563963

4.287981287

0.000194025

0.000818704

0.283437599

MT1G

-1.082597887

12.96743712

-5.150025681

1.85E-05

0.000117877

2.586718443

TUBB3

1.081470203

10.81289795

9.271887653

5.07E-10

4.40E-08

12.98966696

EFHC1

1.08144924

8.498565098

9.503313964

2.99E-10

3.03E-08

13.51153807

MPHOSPH8

1.081120204

8.222430403

6.647821917

3.30E-07

4.67E-06

6.570790457

SLC39A6

1.080520811

8.612275262

5.321066886

1.16E-05

8.09E-05

3.046567941

GMPR2

1.080419808

10.1835269

9.982494926

1.02E-10

1.40E-08

14.56870652

PLPP1

1.079549675

12.22298887

4.588629924

8.57E-05

0.000417251

1.081433867

C3AR1

1.079533021

7.464575983

4.520362847

0.00010324

0.000487288

0.899507206

PKP4

1.078988705

7.219341697

4.508110069

0.00010674

0.000500419

0.86689438

SCG5

1.078692914

6.420091104

3.471874341

0.001699488

0.00500128

-1.81396971

CNOT8

1.078685586

8.779292263

6.264621562

9.09E-07

1.05E-05

5.565445885

MKNK2

1.07867287

9.762189026

11.32712576

5.91E-12

1.88E-09

17.37056083

ZNF721

1.077745797

8.69368259

4.543383862

9.70E-05

0.000462503

0.960815239

STK32B

-1.076764142

8.278102207

-6.487474074

5.03E-07

6.58E-06

6.151743993

DAB2

1.076679738

9.010118077

5.160270103

1.80E-05

0.000115318

2.614262168

WDR1

1.075060578

10.70569774

12.48277575

6.04E-13

4.48E-10

19.59432742

DDX50

1.072849739

10.42516752

5.249666541

1.41E-05

9.46E-05

2.854622248

CLDN3

1.07236717

6.08859028

4.843722356

4.27E-05

0.000233435

1.763928086

ZBTB20

1.071588517

9.17400408

4.031253406

0.000387637

0.001460553

-0.389561913

ASNA1

1.071351132

9.062987359

4.634014168

7.58E-05

0.000376438

1.202572636

COL4A3BP

1.069228532

7.496292481

6.771068462

2.39E-07

3.67E-06

6.891150626

DBT

1.068894976

10.81364504

6.479610391

5.14E-07

6.70E-06

6.131130702

KLF6

1.068595042

8.694058607

3.923383883

0.000517366

0.001851367

-0.669284058

KLHL20

1.068504768

7.038412418

10.21355215

6.17E-11

9.83E-09

15.06727982

EED

1.06808641

8.464217814

7.919227461

1.28E-08

4.24E-07

9.792194251

MTF2

1.067468895

7.289425208

9.731404613

1.79E-10

2.08E-08

14.01867335

USE1

1.067373964

8.080264229

8.78600915

1.57E-09

9.41E-08

11.86994673

DIP2C

-1.064411186

8.287020133

-4.950915171

3.19E-05

0.000184439

2.051618708

GUCY1A3

1.064369955

8.391717113

7.722954073

2.09E-08

5.98E-07

9.307786878

FABP1

-1.063475899

8.139323754

-2.732116105

0.010784021

0.023903519

-3.556239515

NMI

1.063418627

8.913174785

6.061674729

1.56E-06

1.63E-05

5.027985704

FNDC3B

1.062680278

7.298362048

6.320892845

7.83E-07

9.33E-06

5.713893204

C21orf33

1.062481266

7.988234956

3.425838621

0.001914528

0.005546208

-1.92783153

LAP3

1.062459662

10.15323608

5.869684864

2.62E-06

2.47E-05

4.516887066

HSP90B1

1.06186157

8.503549506

5.777879179

3.36E-06

3.01E-05

4.271712257

ING3

1.061456364

7.638573932

4.825058581

4.50E-05

0.000243541

1.71387968

C1QB

1.061188708

6.688390333

3.127770653

0.004091421

0.010523969

-2.649244412

TMBIM6

1.060676668

12.20049452

4.027508929

0.00039155

0.001473553

-0.39930522

GPR161

1.059734032

6.501724924

6.01425427

1.78E-06

1.81E-05

4.901969665

NAA15

1.057880748

6.432962831

6.629466788

3.46E-07

4.86E-06

6.522948198

SULF1

1.05757773

9.843063851

6.231652537

9.93E-07

1.12E-05

5.478350927

WIPI1

1.056510973

8.27825346

6.246765604

9.54E-07

1.09E-05

5.518286309

ZBTB38

1.056169824

7.302859849

7.766411617

1.87E-08

5.53E-07

9.415471696

CSTF1

1.055434663

7.337081595

10.68718219

2.23E-11

4.83E-09

16.06685271

CX3CR1

1.055309203

8.117188378

3.623156632

0.001145417

0.003583227

-1.435762734

NFYC

1.054756655

6.448423523

8.20428046

6.36E-09

2.56E-07

10.48668483

APOL3

1.054037856

10.02998628

7.311646662

5.91E-08

1.28E-06

8.276784737

PALMD

1.053740224

7.735488403

6.012628847

1.78E-06

1.81E-05

4.897647525

CHTOP

1.052486772

7.220378721

6.679260814

3.04E-07

4.38E-06

6.652657347

SEC11A

1.051884002

10.29217746

4.251936424

0.000213903

0.000885124

0.188406866

VAMP5

1.050721926

10.52172068

4.438313791

0.00012905

0.000584474

0.681372633

ANKLE2

1.05042763

7.196105217

8.876060405

1.27E-09

8.10E-08

12.07993162

CAP1

1.050024945

10.95712377

7.506067683

3.61E-08

8.86E-07

8.766758041

P4HB

1.049010802

10.47300414

4.458270788

0.000122236

0.000558178

0.734373822

PLEKHA1

1.048977766

7.19454498

7.575881532

3.02E-08

7.84E-07

8.941559368

SART1

1.047328783

8.1137404

8.376187797

4.19E-09

1.90E-07

10.90025038

ERBB4

-1.046679912

10.46327143

-7.702647823

2.20E-08

6.23E-07

9.257386132

CPQ

1.046010336

10.54802267

5.912742818

2.33E-06

2.24E-05

4.631713331

FXR1

1.045839377

7.714915221

6.751506073

2.51E-07

3.80E-06

6.840405057

NFASC

-1.043772901

11.00079661

-7.995409742

1.06E-08

3.70E-07

9.978857179

RBM8A

1.043747898

7.545132469

4.96501949

3.07E-05

0.00017886

2.089498913

AATF

1.043262199

9.350762714

7.721186391

2.10E-08

6.00E-07

9.303401537

RNF146

1.042920236

7.254295397

5.404368353

9.25E-06

6.72E-05

3.270424275

PTK2

1.042474572

7.441602272

7.416573273

4.52E-08

1.05E-06

8.541792951

NSFL1C

1.042373942

7.852911437

6.47816252

5.16E-07

6.71E-06

6.127334735

FBXW7

1.041338605

7.484615862

5.840734365

2.83E-06

2.63E-05

4.439622073

NSDHL

1.040858898

7.542287566

9.071986684

8.06E-10

6.00E-08

12.53293586

LSM4

1.040785139

7.644681813

7.310557642

5.92E-08

1.28E-06

8.274027254

FBXO9

1.038692099

7.796898194

5.341521258

1.10E-05

7.71E-05

3.101545225

PPARG

1.037896309

6.88201037

7.302296857

6.05E-08

1.30E-06

8.253105673

GLT8D1

1.037533949

8.106135801

7.178182333

8.31E-08

1.64E-06

7.937789238

HMGN4

-1.03747284

9.403636016

-6.593807899

3.80E-07

5.22E-06

6.429909286

PTGER4

1.037231193

6.884584114

5.215070337

1.55E-05

0.000102088

2.761604788

MED6

1.035682324

5.366963402

7.930582401

1.25E-08

4.18E-07

9.820064745

DST

1.034952212

8.875164537

5.651911373

4.72E-06

3.95E-05

3.934617402

IFT27

1.034430229

8.164343121

9.736350061

1.77E-10

2.08E-08

14.02958994

MTUS1

1.033651385

7.914456679

6.513891394

4.69E-07

6.21E-06

6.220950438

ACO2

1.033548604

10.80339959

5.484032163

7.45E-06

5.68E-05

3.484373888

SP3

-1.031245916

9.087202589

-3.605371867

0.001200071

0.003731848

-1.480530029

VDAC3

1.030703765

8.940345624

6.468148347

5.30E-07

6.84E-06

6.101074729

USP39

1.029451601

8.677413981

9.982258621

1.02E-10

1.40E-08

14.56819292

CMTM6

1.02805724

9.525157136

4.774766756

5.16E-05

0.000273084

1.579093631

XYLT1

-1.027723777

8.518221821

-5.498716221

7.16E-06

5.52E-05

3.523792515

ARHGAP19

1.02763613

9.56426488

7.028513894

1.22E-07

2.19E-06

7.55516027

POLR2E

1.027421342

7.924388244

3.308300088

0.002589674

0.00715863

-2.215716541

BTG2

-1.026399219

10.23337218

-7.052038998

1.15E-07

2.10E-06

7.615472284

PRPF19

1.026183615

8.826847728

8.122731764

7.77E-09

2.96E-07

10.2891081

ANKRD11

1.025729915

7.978900966

7.642598687

2.56E-08

6.94E-07

9.108033698

PSMB10

1.024330336

9.421279003

3.339953151

0.002388107

0.006680164

-2.138602883

SRPK2

1.024123484

9.338732967

5.693785151

4.22E-06

3.62E-05

4.04675383

COL4A1

1.024118647

8.992245418

5.104326235

2.10E-05

0.000130711

2.46385594

NRN1

1.024086017

9.095218377

4.759145776

5.38E-05

0.000282366

1.537252436

RSRC2

1.023758956

8.613657235

6.01119626

1.79E-06

1.82E-05

4.893838006

RAC1

1.0232867

11.63257747

4.154851659

0.000278012

0.001103875

-0.066719657

HADHA

1.022601138

10.74473959

6.326166993

7.72E-07

9.21E-06

5.727793185

AUP1

1.021101769

9.246258609

11.719411

2.68E-12

1.22E-09

18.14384431

TXNIP

1.02026765

11.46235662

5.062303643

2.35E-05

0.000143623

2.350896021

TUBB

1.019856466

11.1030305

5.384868531

9.76E-06

6.98E-05

3.218033023

LPL

-1.019304987

9.954464295

-3.318786443

0.002521146

0.006987401

-2.190204228

NPR3

1.019147185

7.765826952

6.380158117

6.69E-07

8.24E-06

5.869949575

GNA11

1.018301382

7.135455751

3.567078203

0.001326501

0.00405132

-1.576653085

ARPC1A

1.018110026

10.05733879

5.427722254

8.68E-06

6.39E-05

3.333160252

TSPAN4

1.017903455

9.153133398

3.286599685

0.002737228

0.007501373

-2.268400912

HNRNPA3

1.017675658

7.627507212

5.584619489

5.67E-06

4.57E-05

3.75426261

JUN

-1.01630629

10.14683512

-8.526608216

2.92E-09

1.45E-07

11.25883533

COBL

-1.016074994

9.359404055

-7.163224111

8.64E-08

1.68E-06

7.899664761

EIF3B

1.015917612

7.916407277

7.295506558

6.16E-08

1.32E-06

8.235902161

COPS6

1.01571967

10.73984087

6.871235397

1.84E-07

3.00E-06

7.150356983

PTPRO

-1.015461858

12.12826016

-4.113981359

0.000310368

0.001211407

-0.173730401

ZFP36

-1.015055803

9.582243452

-6.92601257

1.59E-07

2.71E-06

7.2916514

BUD23

1.014932236

9.485752254

6.221938523

1.02E-06

1.15E-05

5.452672596

PBLD

-1.014740962

10.58094791

-3.724016801

0.000878415

0.002881032

-1.180450248

SNTB2

1.014417654

7.961457348

6.282527181

8.67E-07

1.01E-05

5.612710524

CTDSPL

1.01439347

10.43635804

4.266936434

0.000205397

0.000857252

0.22793438

RNF7

1.01409318

9.590065207

9.476640922

3.18E-10

3.19E-08

13.45176623

PTPRN2

1.013187332

9.817821348

9.100558946

7.54E-10

5.75E-08

12.59855544

ZBTB18

-1.012739735

9.549857266

-6.564108062

4.11E-07

5.59E-06

6.352323976

SERINC3

1.012252699

9.515098643

5.004169961

2.76E-05

0.000164243

2.194671288

EPHA4

1.012059148

7.692051937

8.221126207

6.11E-09

2.48E-07

10.52738762

EXPH5

-1.011374294

9.267532038

-6.395702776

6.42E-07

7.99E-06

5.910831112

SLC14A1

1.010232523

8.216419273

4.925155609

3.42E-05

0.00019565

1.982449863

AMPH

-1.008900528

7.734124024

-3.684116541

0.000975864

0.003135087

-1.281735767

HLA-J

1.00871551

10.19609554

8.597452642

2.46E-09

1.28E-07

11.42665058

PRMT5

1.007933207

9.428092233

7.939886786

1.22E-08

4.11E-07

9.842889558

PON2

1.007800835

10.09079366

3.81949425

0.000682209

0.002328987

-0.936662463

FBXW2

1.007654936

7.868340028

7.861953607

1.48E-08

4.63E-07

9.651359007

STX18

1.006898222

10.37920307

10.5061697

3.28E-11

6.42E-09

15.68836623

MRPL9

1.00575951

8.56785435

10.39823871

4.14E-11

7.62E-09

15.4606155

NCOA1

1.005421619

9.05825609

6.916782293

1.63E-07

2.76E-06

7.267865336

EIF3D

1.004960463

10.76774901

9.588105769

2.47E-10

2.61E-08

13.70089836

ZNF45

1.004939996

5.556368284

6.771883066

2.38E-07

3.67E-06

6.893262879

RBL2

1.004173461

7.21346767

8.632505051

2.27E-09

1.21E-07

11.50942809

PTP4A2

1.003941839

7.140081341

8.279182405

5.30E-09

2.24E-07

10.66737061

CXADR

1.003893604

9.64625798

3.484927272

0.001642901

0.004854007

-1.781577202

TTC37

1.001622392

7.444769781

5.465907256

7.83E-06

5.91E-05

3.435710333

ATP6V0E2

-1.001198901

9.83254098

-7.451280242

4.14E-08

9.82E-07

8.629154547

UROD

1.001043527

9.325183612

16.03116183

1.28E-15

9.51E-12

25.49653811

THSD7A

1.001019085

9.626288581

3.889413906

0.00056643

0.001995535

-0.756941013

NFIL3

-1.000765264

8.473403841

-5.219220674

1.53E-05

0.000101131

2.77276385

POLR2A

-1.000463151

8.643383429

-6.408934128

6.20E-07

7.77E-06

5.945612006

Article Type

Research Article

Publication history

Received: October 29, 2019
Accepted: November 11, 2019
Published: November 15, 2019

Citation

Qianhong Ma, Zhang D, Huang Z (2019) Novel Pathology-Related Hub Genes in Focal Segmental Glomerulosclerosis. J Clin Res Med Volume 2 (5): 1–10. DOI: 10.31038/JCRM.2019252

Corresponding author

Zhongying Huang
West China Second Hospital,
Sichuan University,
Chengdu, Sichuan,
Zipcode: 610041, China;
China; Tel: 86-28-85501650;
E-mail: huangzyhxyd@scu.edu.cn;